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package Bio::Roary::CombinedProteome; |
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$Bio::Roary::CombinedProteome::VERSION = '3.11.0'; |
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# ABSTRACT: Take in multiple FASTA sequences containing proteomes and concat them together and output a FASTA file, filtering out more than 5% X's |
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use Moose; |
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use Bio::Roary::Exceptions; |
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has 'proteome_files' => ( is => 'ro', isa => 'ArrayRef', required => 1 ); |
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has 'output_filename' => ( is => 'ro', isa => 'Str', default => 'combined_output.fa' ); |
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sub BUILD { |
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my ($self) = @_; |
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for my $filename ( @{ $self->proteome_files } ) { |
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Bio::Roary::Exceptions::FileNotFound->throw( error => 'Cant open file: ' . $filename ) |
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unless ( -e $filename ); |
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} |
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} |
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sub create_combined_proteome_file { |
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my ($self) = @_; |
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unlink($self->output_filename); |
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for my $filename (@{$self->proteome_files }) |
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{ |
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system(join(' ', ("cat", $filename, ">>", $self->output_filename))); |
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} |
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1; |
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} |
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no Moose; |
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__PACKAGE__->meta->make_immutable; |
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1; |
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__END__ |
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=pod |
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=encoding UTF-8 |
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=head1 NAME |
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Bio::Roary::CombinedProteome - Take in multiple FASTA sequences containing proteomes and concat them together and output a FASTA file, filtering out more than 5% X's |
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=head1 VERSION |
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version 3.11.0 |
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=head1 SYNOPSIS |
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Take in multiple FASTA sequences containing proteomes and concat them together and output a FASTA file, filtering out more than 5% X's |
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use Bio::Roary::CombinedProteome; |
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my $obj = Bio::Roary::CombinedProteome->new( |
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proteome_files => ['abc.fa','efg.fa'], |
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output_filename => 'example_output.fa', |
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maximum_percentage_of_unknowns => 5.0, |
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); |
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$obj->create_combined_proteome_file; |
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=head1 AUTHOR |
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Andrew J. Page <ap13@sanger.ac.uk> |
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=head1 COPYRIGHT AND LICENSE |
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This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. |
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This is free software, licensed under: |
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The GNU General Public License, Version 3, June 2007 |
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=cut |