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package Bio::Roary::ClustersRole; |
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# ABSTRACT: A role to read a clusters file from CD hit |
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$Bio::Roary::ClustersRole::VERSION = '3.11.0'; |
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use Moose::Role; |
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use Bio::Roary::Exceptions; |
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has 'clusters_filename' => ( is => 'ro', isa => 'Str', required => 1 ); |
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has '_clustered_genes' => ( is => 'ro',lazy => 1, builder => '_build__clustered_genes' ); |
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has '_clusters_fh' => ( is => 'ro',lazy => 1, builder => '_build__clusters_fh' ); |
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sub _build__clusters_fh |
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{ |
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my($self) = @_; |
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open(my $fh, $self->clusters_filename) or Bio::Roary::Exceptions::FileNotFound->throw( error => 'Cant open file: ' . $self->clusters_filename ); |
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return $fh; |
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} |
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sub _build__clustered_genes |
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{ |
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my($self) = @_; |
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my $fh = $self->_clusters_fh; |
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my %clustered_genes ; |
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my %raw_clusters; |
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my $current_cluster_name; |
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while(<$fh>) |
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{ |
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my $line = $_; |
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if($line =~ /^>(.+)$/) |
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{ |
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$current_cluster_name = $1; |
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} |
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#>Cluster 5 |
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#0 4201aa, >6630_4#9_00008... * |
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#1 4201aa, >6631_1#23_00379... at 100.00% |
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if($line =~ /[\d]+\t[\w]+, >(.+)\.\.\. (.+)$/) |
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{ |
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my $gene_name = $1; |
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my $identity = $2; |
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if($identity eq '*') |
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{ |
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$raw_clusters{$current_cluster_name}{representative_gene_name} = $gene_name; |
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} |
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else |
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{ |
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push(@{$raw_clusters{$current_cluster_name}{gene_names}}, $gene_name); |
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} |
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} |
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} |
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# iterate over the raw clusters and convert to a simple hash |
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for my $cluster_name (keys %raw_clusters) |
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{ |
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$clustered_genes{$raw_clusters{$cluster_name}{representative_gene_name}} = $raw_clusters{$cluster_name}{gene_names}; |
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} |
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return \%clustered_genes; |
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} |
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1; |
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__END__ |
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=pod |
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=encoding UTF-8 |
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=head1 NAME |
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Bio::Roary::ClustersRole - A role to read a clusters file from CD hit |
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=head1 VERSION |
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version 3.11.0 |
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=head1 SYNOPSIS |
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A role to read a clusters file from CD hit |
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with 'Bio::Roary::ClustersRole'; |
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=head1 AUTHOR |
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Andrew J. Page <ap13@sanger.ac.uk> |
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=head1 COPYRIGHT AND LICENSE |
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This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. |
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This is free software, licensed under: |
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The GNU General Public License, Version 3, June 2007 |
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=cut |