line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
1
|
|
|
|
|
|
|
package Bio::Roary::Output::BlastIdentityFrequency; |
2
|
|
|
|
|
|
|
$Bio::Roary::Output::BlastIdentityFrequency::VERSION = '3.10.2'; |
3
|
|
|
|
|
|
|
# ABSTRACT: Take in blast results and find the percentage identity graph |
4
|
|
|
|
|
|
|
|
5
|
|
|
|
|
|
|
|
6
|
1
|
|
|
1
|
|
7
|
use Moose; |
|
1
|
|
|
|
|
2
|
|
|
1
|
|
|
|
|
8
|
|
7
|
1
|
|
|
1
|
|
6007
|
use Bio::SeqIO; |
|
1
|
|
|
|
|
2
|
|
|
1
|
|
|
|
|
21
|
|
8
|
1
|
|
|
1
|
|
4
|
use Bio::Roary::Exceptions; |
|
1
|
|
|
|
|
1
|
|
|
1
|
|
|
|
|
236
|
|
9
|
|
|
|
|
|
|
|
10
|
|
|
|
|
|
|
has 'input_filename' => ( is => 'ro', isa => 'Str', default => '_blast_results' ); |
11
|
|
|
|
|
|
|
has 'output_filename' => ( is => 'ro', isa => 'Str', default => 'blast_identity_frequency.Rtab' ); |
12
|
|
|
|
|
|
|
|
13
|
|
|
|
|
|
|
has '_output_fh' => ( is => 'ro', lazy => 1, builder => '_build__output_fh' ); |
14
|
|
|
|
|
|
|
has '_input_fh' => ( is => 'ro', lazy => 1, builder => '_build__input_fh' ); |
15
|
|
|
|
|
|
|
|
16
|
|
|
|
|
|
|
sub _build__output_fh |
17
|
|
|
|
|
|
|
{ |
18
|
0
|
|
|
0
|
|
|
my ($self) = @_; |
19
|
0
|
0
|
|
|
|
|
open( my $fh, '>', $self->output_filename ) |
20
|
|
|
|
|
|
|
or Bio::Roary::Exceptions::CouldntWriteToFile->throw( |
21
|
|
|
|
|
|
|
error => "Couldnt write output file:" . $self->output_filename ); |
22
|
0
|
|
|
|
|
|
return $fh; |
23
|
|
|
|
|
|
|
} |
24
|
|
|
|
|
|
|
|
25
|
|
|
|
|
|
|
sub _build__input_fh |
26
|
|
|
|
|
|
|
{ |
27
|
0
|
|
|
0
|
|
|
my ($self) = @_; |
28
|
0
|
|
|
|
|
|
my $input_string = 'awk \'{print $3}\' '.$self->input_filename.' | awk \'BEGIN {FS="."}; {print $1}\'| sort | uniq -c | awk \'{print $2"\t"$1}\''; |
29
|
|
|
|
|
|
|
|
30
|
0
|
0
|
|
|
|
|
open( my $fh, '-|', $input_string ) or die "Couldnt open results file"; |
31
|
0
|
|
|
|
|
|
return $fh; |
32
|
|
|
|
|
|
|
} |
33
|
|
|
|
|
|
|
|
34
|
|
|
|
|
|
|
sub create_file |
35
|
|
|
|
|
|
|
{ |
36
|
0
|
|
|
0
|
0
|
|
my ($self) = @_; |
37
|
|
|
|
|
|
|
|
38
|
0
|
|
|
|
|
|
my $input_fh = $self->_input_fh; |
39
|
0
|
|
|
|
|
|
while(<$input_fh>) |
40
|
|
|
|
|
|
|
{ |
41
|
0
|
|
|
|
|
|
print {$self->_output_fh} $_; |
|
0
|
|
|
|
|
|
|
42
|
|
|
|
|
|
|
} |
43
|
0
|
|
|
|
|
|
close($self->_input_fh); |
44
|
0
|
|
|
|
|
|
close($self->_output_fh); |
45
|
|
|
|
|
|
|
} |
46
|
|
|
|
|
|
|
|
47
|
1
|
|
|
1
|
|
6
|
no Moose; |
|
1
|
|
|
|
|
2
|
|
|
1
|
|
|
|
|
5
|
|
48
|
|
|
|
|
|
|
__PACKAGE__->meta->make_immutable; |
49
|
|
|
|
|
|
|
|
50
|
|
|
|
|
|
|
1; |
51
|
|
|
|
|
|
|
|
52
|
|
|
|
|
|
|
__END__ |
53
|
|
|
|
|
|
|
|
54
|
|
|
|
|
|
|
=pod |
55
|
|
|
|
|
|
|
|
56
|
|
|
|
|
|
|
=encoding UTF-8 |
57
|
|
|
|
|
|
|
|
58
|
|
|
|
|
|
|
=head1 NAME |
59
|
|
|
|
|
|
|
|
60
|
|
|
|
|
|
|
Bio::Roary::Output::BlastIdentityFrequency - Take in blast results and find the percentage identity graph |
61
|
|
|
|
|
|
|
|
62
|
|
|
|
|
|
|
=head1 VERSION |
63
|
|
|
|
|
|
|
|
64
|
|
|
|
|
|
|
version 3.10.2 |
65
|
|
|
|
|
|
|
|
66
|
|
|
|
|
|
|
=head1 SYNOPSIS |
67
|
|
|
|
|
|
|
|
68
|
|
|
|
|
|
|
Take in blast results and find the percentage identity graph |
69
|
|
|
|
|
|
|
use Bio::Roary::Output::BlastIdentityFrequency; |
70
|
|
|
|
|
|
|
|
71
|
|
|
|
|
|
|
my $obj = Bio::Roary::Output::BlastIdentityFrequency->new( |
72
|
|
|
|
|
|
|
input_filename => '_blast_results', |
73
|
|
|
|
|
|
|
output_filename => 'blast_identity_frequency.Rtab', |
74
|
|
|
|
|
|
|
); |
75
|
|
|
|
|
|
|
$obj->create_file(); |
76
|
|
|
|
|
|
|
|
77
|
|
|
|
|
|
|
=head1 AUTHOR |
78
|
|
|
|
|
|
|
|
79
|
|
|
|
|
|
|
Andrew J. Page <ap13@sanger.ac.uk> |
80
|
|
|
|
|
|
|
|
81
|
|
|
|
|
|
|
=head1 COPYRIGHT AND LICENSE |
82
|
|
|
|
|
|
|
|
83
|
|
|
|
|
|
|
This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. |
84
|
|
|
|
|
|
|
|
85
|
|
|
|
|
|
|
This is free software, licensed under: |
86
|
|
|
|
|
|
|
|
87
|
|
|
|
|
|
|
The GNU General Public License, Version 3, June 2007 |
88
|
|
|
|
|
|
|
|
89
|
|
|
|
|
|
|
=cut |