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package Bio::Roary::External::IterativeCdhit; |
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$Bio::Roary::External::IterativeCdhit::VERSION = '3.10.2'; |
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# ABSTRACT: Iteratively run CDhit |
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use Moose; |
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with 'Bio::Roary::JobRunner::Role'; |
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has 'output_cd_hit_filename' => ( is => 'ro', isa => 'Str', required => 1 ); |
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has 'output_combined_filename' => ( is => 'ro', isa => 'Str', required => 1 ); |
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has 'number_of_input_files' => ( is => 'ro', isa => 'Int', required => 1 ); |
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has 'output_filtered_clustered_fasta' => ( is => 'ro', isa => 'Str', required => 1 ); |
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has 'exec' => ( is => 'ro', isa => 'Str', default => 'iterative_cdhit' ); |
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has '_max_cpus' => ( is => 'ro', isa => 'Int', default => 40 ); |
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# Overload Role |
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has 'memory_in_mb' => ( is => 'ro', isa => 'Int', lazy => 1, builder => '_build_memory_in_mb' ); |
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sub _build_memory_in_mb { |
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my ($self) = @_; |
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my $filename = $self->output_combined_filename; |
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my $memory_required = 2000; |
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if ( -e $filename ) { |
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$memory_required = -s $filename; |
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# Convert to mb |
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$memory_required = int( $memory_required / 1000000 ); |
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# Pentuple memory for worst case senario |
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$memory_required *= 5; |
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$memory_required = 2000 if ( $memory_required < 2000 ); |
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} |
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return $memory_required; |
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} |
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sub _build__max_available_memory_in_mb { |
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my ($self) = @_; |
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my $memory_to_cdhit = int( $self->memory_in_mb * 0.9 ); |
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return $memory_to_cdhit; |
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} |
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sub _command_to_run { |
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my ($self) = @_; |
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my $cpus = ($self->cpus > $self->_max_cpus) ? $self->_max_cpus : $self->cpus; |
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return join( |
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' ', |
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( |
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$self->exec, '-c', $self->output_cd_hit_filename, '-m', |
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$self->output_combined_filename, '-n', $self->number_of_input_files, '--cpus', $cpus, '-f', |
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$self->output_filtered_clustered_fasta |
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) |
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); |
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} |
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sub run { |
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my ($self) = @_; |
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my @commands_to_run; |
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push( @commands_to_run, $self->_command_to_run ); |
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$self->logger->info( "Running command: " . $self->_command_to_run() ); |
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my $job_runner_obj = $self->_job_runner_class->new( |
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commands_to_run => \@commands_to_run, |
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memory_in_mb => $self->memory_in_mb, |
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queue => $self->_queue, |
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cpus => $self->cpus |
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); |
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$job_runner_obj->run(); |
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1; |
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} |
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6293
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no Moose; |
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__PACKAGE__->meta->make_immutable; |
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1; |
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__END__ |
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=pod |
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=encoding UTF-8 |
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=head1 NAME |
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Bio::Roary::External::IterativeCdhit - Iteratively run CDhit |
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=head1 VERSION |
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version 3.10.2 |
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=head1 SYNOPSIS |
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Iteratively run CDhit |
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use Bio::Roary::External::IterativeCdhit; |
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my $seg= Bio::Roary::External::IterativeCdhit->new( |
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output_cd_hit_filename => '', |
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output_combined_filename => '', |
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number_of_input_files => 10, |
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output_filtered_clustered_fasta => '', |
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); |
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$seg->run(); |
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=head1 AUTHOR |
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Andrew J. Page <ap13@sanger.ac.uk> |
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=head1 COPYRIGHT AND LICENSE |
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This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. |
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This is free software, licensed under: |
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The GNU General Public License, Version 3, June 2007 |
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=cut |