139
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140
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141
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Set $num |
142
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143
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| Primer |
144
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| Sequence |
145
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| Tm |
146
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| Coordinate |
147
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| Primer Pair Quality |
148
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149
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"; |
150
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} |
151
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152
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# ------------------------------------------------------------------- |
153
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sub PCR_row { |
154
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155
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=head2 PCR_row |
156
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157
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Returns table rows with PCR primer info. Should be called once for |
158
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each primer pair. Expects to be passed a hash containing the |
159
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Bio::PrimerDesigner::Result object and the primer set number and an |
160
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(optional) label. |
161
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162
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my $pcr_row = PCR_row( |
163
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primers => $result_obj, |
164
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setnum => $set_number, |
165
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label => $label |
166
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); |
167
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168
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=cut |
169
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170
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1
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1
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1
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2
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my $self = shift; |
171
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1
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50
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7
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my %primers = @_ or return $self->error('No arguments for PCR_row method'); |
172
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1
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50
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7
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my $set = $primers{'setnum'} || 1; |
173
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1
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50
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6
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my $label = $primers{'label'} || 1; |
174
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1
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2
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my %args = %{$primers{'primers'}{$set}}; |
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1
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209
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175
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176
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0
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return " |
177
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178
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| $label |
179
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| Forward |
180
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| $args{'left'} |
181
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| $args{'tmleft'} |
182
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| $args{'startleft'} |
183
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| |
184
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185
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186
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| |
187
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| Reverse |
188
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| $args{'right'} |
189
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| $args{'tmright'} |
190
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| $args{'startright'} |
191
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| $args{'qual'} |
192
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193
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"; |
194
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} |
195
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196
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# ------------------------------------------------------------------- |
197
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sub ePCR_row { |
198
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199
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=head2 ePCR_row |
200
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201
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Returns table rows summarizing e-PCR results. Expects to be passed an |
202
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Bio::PrimerDesigner::Result e-PCR results object and an optional e-PCR label. |
203
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204
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=cut |
205
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206
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0
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0
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1
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my $self = shift; |
207
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0
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0
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|
my $args = shift or return $self->error('No arguments for ePCR_row method'); |
208
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0
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my %epcr = %$args; |
209
|
0
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|
my $label = shift; |
210
|
0
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|
my $num_prods = $epcr{1}{'products'}; |
211
|
0
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0
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|
my $s = $num_prods > 1 ? 's' : ''; |
212
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0
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0
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|
$num_prods ||= 'No'; |
213
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0
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my $sizes = ''; |
214
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215
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0
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for (1..$num_prods) { |
216
|
0
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0
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|
if ($_ == 1) { |
|
|
0
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217
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0
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|
$sizes = "Size$s $epcr{$_}{'size'}" |
218
|
|
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|
} |
219
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|
elsif ($_ < $num_prods) { |
220
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0
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|
$sizes .= ", " . $epcr{$_}{'size'} |
221
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|
} |
222
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else { |
223
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0
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|
$sizes .= "and " . $epcr{$_}{'size'} |
224
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} |
225
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} |
226
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0
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0
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|
|
$sizes .= " bp" if $num_prods ne 'No'; |
227
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0
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|
my $row = " |
228
|
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|
229
|
|
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|
|
|
230
|
|
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|
|
$label e-PCR Results: |
231
|
|
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|
|
$num_prods product${s} |
232
|
|
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233
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|
$sizes |
234
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235
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236
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237
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"; |
238
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239
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0
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|
|
$row; |
240
|
|
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|
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|
|
} |
241
|
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|
242
|
|
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|
|
|
|
# ------------------------------------------------------------------- |
243
|
|
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|
|
|
sub render { |
244
|
|
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245
|
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|
|
=head2 render |
246
|
|
|
|
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247
|
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|
|
Renders the image URL. Expects to be passed a hash of the map start |
248
|
|
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|
|
|
|
and stop, and other features to be mapped (i.e. |
249
|
|
|
|
|
|
|
gene,forward_primer,reverse_primer, label,start and stop of each |
250
|
|
|
|
|
|
|
feature, and gene strand). |
251
|
|
|
|
|
|
|
|
252
|
|
|
|
|
|
|
my $image = $page->render( |
253
|
|
|
|
|
|
|
start => $startleft, |
254
|
|
|
|
|
|
|
stop => $startright, |
255
|
|
|
|
|
|
|
feat => $features |
256
|
|
|
|
|
|
|
); |
257
|
|
|
|
|
|
|
|
258
|
|
|
|
|
|
|
=cut |
259
|
|
|
|
|
|
|
|
260
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
261
|
0
|
0
|
|
|
|
|
my %draw = @_ or return $self->error('No name argument'); |
262
|
0
|
|
0
|
|
|
|
my $start = $draw{'start'} || 0; |
263
|
0
|
|
0
|
|
|
|
my $stop = $draw{'stop'} || 0; |
264
|
0
|
|
0
|
|
|
|
my $feat = $draw{'feat'} || ''; |
265
|
|
|
|
|
|
|
|
266
|
0
|
|
|
|
|
|
(my $config = <<"END") =~ s/^\s+//gm; |
267
|
|
|
|
|
|
|
[general] |
268
|
|
|
|
|
|
|
bases = $start-$stop |
269
|
|
|
|
|
|
|
height = 12 |
270
|
|
|
|
|
|
|
|
271
|
|
|
|
|
|
|
[gene] |
272
|
|
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|
|
|
|
glyph = transcript2 |
273
|
|
|
|
|
|
|
bgcolor = cyan |
274
|
|
|
|
|
|
|
label = 1 |
275
|
|
|
|
|
|
|
description = 1 |
276
|
|
|
|
|
|
|
height = 7 |
277
|
|
|
|
|
|
|
|
278
|
|
|
|
|
|
|
[forward_primer] |
279
|
|
|
|
|
|
|
glyph = triangle |
280
|
|
|
|
|
|
|
bgcolor = blue |
281
|
|
|
|
|
|
|
orient = E |
282
|
|
|
|
|
|
|
height = 7 |
283
|
|
|
|
|
|
|
label = 1 |
284
|
|
|
|
|
|
|
|
285
|
|
|
|
|
|
|
[reverse_primer] |
286
|
|
|
|
|
|
|
glyph = triangle |
287
|
|
|
|
|
|
|
bgcolor = green |
288
|
|
|
|
|
|
|
orient = W |
289
|
|
|
|
|
|
|
height = 7 |
290
|
|
|
|
|
|
|
label = 1 |
291
|
|
|
|
|
|
|
|
292
|
|
|
|
|
|
|
$feat |
293
|
|
|
|
|
|
|
END |
294
|
|
|
|
|
|
|
|
295
|
0
|
|
|
|
|
|
$config =~ s/\n/@@/gm; |
296
|
0
|
|
|
|
|
|
$config =~ s/\s+/%20/g; |
297
|
|
|
|
|
|
|
|
298
|
0
|
|
|
|
|
|
$config = "
|
299
|
|
|
|
|
|
|
"perl/render?width=700;text=\'$config\'\">"; |
300
|
0
|
|
|
|
|
|
return $config; |
301
|
|
|
|
|
|
|
} |
302
|
|
|
|
|
|
|
|
303
|
|
|
|
|
|
|
# ------------------------------------------------------------------- |
304
|
|
|
|
|
|
|
sub PCR_map { |
305
|
|
|
|
|
|
|
|
306
|
|
|
|
|
|
|
=head2 PCR_map |
307
|
|
|
|
|
|
|
|
308
|
|
|
|
|
|
|
Returns a 6 column wide table cell with the info. |
309
|
|
|
|
|
|
|
Will display the image of mapped primers in the browser when |
310
|
|
|
|
|
|
|
passed the image URL. |
311
|
|
|
|
|
|
|
|
312
|
|
|
|
|
|
|
=cut |
313
|
|
|
|
|
|
|
|
314
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
315
|
0
|
|
0
|
|
|
|
my $image_url = shift || ''; |
316
|
|
|
|
|
|
|
|
317
|
0
|
|
|
|
|
|
return " |
318
|
|
|
|
|
|
|
|
319
|
|
|
|
|
|
|
| |
320
|
|
|
|
|
|
|
$image_url |
321
|
|
|
|
|
|
|
|
322
|
|
|
|
|
|
|
|
323
|
|
|
|
|
|
|
|
324
|
|
|
|
|
|
|
| |
325
|
|
|
|
|
|
|
|
326
|
|
|
|
|
|
|
"; |
327
|
|
|
|
|
|
|
} |
328
|
|
|
|
|
|
|
|
329
|
|
|
|
|
|
|
1; |
330
|
|
|
|
|
|
|
|
331
|
|
|
|
|
|
|
# ------------------------------------------------------------------- |
332
|
|
|
|
|
|
|
|
333
|
|
|
|
|
|
|
=pod |
334
|
|
|
|
|
|
|
|
335
|
|
|
|
|
|
|
=head1 AUTHOR |
336
|
|
|
|
|
|
|
|
337
|
|
|
|
|
|
|
Copyright (C) 2003-2009 Sheldon McKay Emckays@cshl.eduE. |
338
|
|
|
|
|
|
|
|
339
|
|
|
|
|
|
|
=head1 LICENSE |
340
|
|
|
|
|
|
|
|
341
|
|
|
|
|
|
|
This program is free software; you can redistribute it and/or modify |
342
|
|
|
|
|
|
|
it under the terms of the GNU General Public License as published by |
343
|
|
|
|
|
|
|
the Free Software Foundation; version 3 or any later version. |
344
|
|
|
|
|
|
|
|
345
|
|
|
|
|
|
|
This program is distributed in the hope that it will be useful, but |
346
|
|
|
|
|
|
|
WITHOUT ANY WARRANTY; without even the implied warranty of |
347
|
|
|
|
|
|
|
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU |
348
|
|
|
|
|
|
|
General Public License for more details. |
349
|
|
|
|
|
|
|
|
350
|
|
|
|
|
|
|
You should have received a copy of the GNU General Public License |
351
|
|
|
|
|
|
|
along with this program; if not, write to the Free Software |
352
|
|
|
|
|
|
|
Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 |
353
|
|
|
|
|
|
|
USA. |
354
|
|
|
|
|
|
|
|
355
|
|
|
|
|
|
|
=head1 SEE ALSO |
356
|
|
|
|
|
|
|
|
357
|
|
|
|
|
|
|
Bio::PrimerDesigner::primer3, Bio::PrimerDesigner::epcr. |
358
|
|
|
|
|
|
|
|
359
|
|
|
|
|
|
|
=cut |