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package Bio::Phylo::Matrices::DatumRole; |
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use strict; |
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374
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use Bio::Phylo::Util::MOP; |
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105
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67
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use base qw'Bio::Phylo::Matrices::TypeSafeData Bio::Phylo::Taxa::TaxonLinker'; |
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5111
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use Bio::Phylo::Util::OptionalInterface 'Bio::Seq'; |
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94
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6
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use Bio::Phylo::Util::Exceptions 'throw'; |
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695
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use Bio::Phylo::Util::CONSTANT qw':objecttypes /looks_like/'; |
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3408
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use Bio::Phylo::NeXML::Writable; |
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use Bio::Phylo::Factory; |
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my $LOADED_WRAPPERS = 0; |
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{ |
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my $fac = Bio::Phylo::Factory->new; |
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my $logger = __PACKAGE__->get_logger; |
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my $TYPE_CONSTANT = _DATUM_; |
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my $CONTAINER_CONSTANT = _MATRIX_; |
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#{ |
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#my @fields = \( my ( %weight, %position, %annotations ) ); |
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#} |
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=head1 NAME |
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Bio::Phylo::Matrices::DatumRole - Extra behaviours for a character state sequence |
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24
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=head1 SYNOPSIS |
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use Bio::Phylo::Factory; |
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my $fac = Bio::Phylo::Factory->new; |
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29
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# instantiating a datum object... |
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my $datum = $fac->create_datum( |
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-name => 'Tooth comb size, |
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-type => 'STANDARD', |
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-desc => 'number of teeth in lower jaw comb', |
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-pos => 1, |
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-weight => 2, |
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-char => [ 6 ], |
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); |
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39
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# ...and linking it to a taxon object |
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my $taxon = $fac->create_taxon( |
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-name => 'Lemur_catta' |
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); |
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$datum->set_taxon( $taxon ); |
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45
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# instantiating a matrix... |
46
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my $matrix = $fac->create_matrix; |
47
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48
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# ...and insert datum in matrix |
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$matrix->insert($datum); |
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51
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=head1 DESCRIPTION |
52
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53
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The datum object models a single observation or a sequence of observations, |
54
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which can be linked to a taxon object. |
55
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56
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=head1 METHODS |
57
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58
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=head2 CONSTRUCTOR |
59
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60
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=over |
61
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62
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=item new() |
63
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64
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Datum object constructor. |
65
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66
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Type : Constructor |
67
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Title : new |
68
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Usage : my $datum = Bio::Phylo::Matrices::Datum->new; |
69
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Function: Instantiates a Bio::Phylo::Matrices::Datum |
70
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object. |
71
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Returns : A Bio::Phylo::Matrices::Datum object. |
72
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Args : None required. Optional: |
73
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-taxon => $taxon, |
74
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-weight => 0.234, |
75
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-type => DNA, |
76
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-pos => 2, |
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78
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=cut |
79
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80
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sub new : Constructor { |
81
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82
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# could be child class |
83
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724
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724
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1
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2456
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my $class = shift; |
84
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85
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# notify user |
86
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724
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5433
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$logger->info("constructor called for '$class'"); |
87
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724
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100
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2591
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if ( not $LOADED_WRAPPERS ) { |
88
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14
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0
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179
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eval do { local $/; <DATA> }; |
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89
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14
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50
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389
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die $@ if $@; |
90
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14
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36
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$LOADED_WRAPPERS++; |
91
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} |
92
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93
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# go up inheritance tree, eventually get an ID |
94
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724
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4342
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my $self = $class->SUPER::new( |
95
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@_, |
96
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'-listener' => \&_update_characters, |
97
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); |
98
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724
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3651
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return $self; |
99
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16
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98
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} |
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16
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29
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16
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74
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100
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101
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=item new_from_bioperl() |
102
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103
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Datum constructor from Bio::Seq argument. |
104
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105
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Type : Constructor |
106
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Title : new_from_bioperl |
107
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Usage : my $datum = |
108
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Bio::Phylo::Matrices::Datum->new_from_bioperl($seq); |
109
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Function: Instantiates a |
110
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Bio::Phylo::Matrices::Datum object. |
111
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Returns : A Bio::Phylo::Matrices::Datum object. |
112
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Args : A Bio::Seq (or similar) object |
113
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114
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=cut |
115
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116
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sub new_from_bioperl { |
117
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0
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0
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1
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0
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my ( $class, $seq, @args ) = @_; |
118
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119
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# want $seq type-check here? Allowable: is-a Bio::PrimarySeq, |
120
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# Bio::LocatableSeq /maj |
121
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0
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0
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0
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my $type = $seq->alphabet || $seq->_guess_alphabet || 'dna'; |
122
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0
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0
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my $self = $class->new( '-type' => $type, @args ); |
123
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124
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# copy seq string |
125
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0
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0
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my $seqstring = $seq->seq; |
126
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0
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0
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0
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0
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if ( $seqstring and $seqstring =~ /\S/ ) { |
127
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0
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0
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eval { $self->set_char($seqstring) }; |
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128
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0
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if ( |
129
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$@ |
130
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and looks_like_instance( |
131
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$@, 'Bio::Phylo::Util::Exceptions::InvalidData' |
132
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) |
133
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) |
134
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{ |
135
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0
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0
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$logger->error( |
136
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"\nAn exception of type Bio::Phylo::Util::Exceptions::InvalidData was caught\n\n" |
137
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. $@->description |
138
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. "\n\nThe BioPerl sequence object contains invalid data ($seqstring)\n" |
139
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. "I cannot store this string, I will continue instantiating an empty object.\n" |
140
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. "---------------------------------- STACK ----------------------------------\n" |
141
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. $@->trace->as_string |
142
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. "\n--------------------------------------------------------------------------" |
143
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); |
144
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} |
145
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} |
146
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147
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# copy name |
148
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0
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0
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my $name = $seq->display_id; |
149
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0
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0
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$self->set_name($name) if defined $name; |
150
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151
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# copy desc |
152
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0
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0
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my $desc = $seq->desc; |
153
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0
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0
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0
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$self->set_desc($desc) if defined $desc; |
154
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155
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# only Bio::LocatableSeq objs have these fields... |
156
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0
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0
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for my $field (qw(start end strand)) { |
157
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0
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0
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0
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$self->$field( $seq->$field ) if $seq->can($field); |
158
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} |
159
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0
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0
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return $self; |
160
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} |
161
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162
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=back |
163
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164
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=head2 MUTATORS |
165
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166
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=over |
167
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168
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=item set_char() |
169
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170
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Sets character state(s) |
171
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172
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Type : Mutator |
173
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Title : set_char |
174
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Usage : $datum->set_char($char); |
175
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Function: Assigns a datum's character value. |
176
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Returns : Modified object. |
177
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Args : The $char argument is checked against |
178
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the allowed ranges for the various |
179
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character types: IUPAC nucleotide (for |
180
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types of DNA|RNA|NUCLEOTIDE), IUPAC |
181
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single letter amino acid codes (for type |
182
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PROTEIN), integers (STANDARD) or any of perl's |
183
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decimal formats (CONTINUOUS). The $char can be: |
184
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* a single character; |
185
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* a string of characters; |
186
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* an array reference of characters; |
187
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* an array of characters; |
188
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|
Comments: Note that on assigning characters to a datum, |
189
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previously set annotations are removed. |
190
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191
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=cut |
192
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193
|
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|
sub set_char { |
194
|
713
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|
713
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1
|
1616
|
my $self = shift; |
195
|
713
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|
3036
|
my $name = $self->get_internal_name; |
196
|
713
|
100
|
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|
3170
|
my $length = ref $_[0] ? join( '', @{ $_[0] } ) : join( '', @_ ); |
|
69
|
|
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|
163
|
|
197
|
713
|
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|
5591
|
$logger->info("setting $name $length chars '@_'"); |
198
|
713
|
|
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|
1568
|
my @data; |
199
|
713
|
|
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|
1879
|
for my $arg (@_) { |
200
|
713
|
100
|
|
|
|
2828
|
if ( looks_like_instance( $arg, 'ARRAY' ) ) { |
201
|
69
|
|
|
|
|
99
|
push @data, @{$arg}; |
|
69
|
|
|
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|
230
|
|
202
|
|
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|
|
} |
203
|
|
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|
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|
|
else { |
204
|
644
|
|
|
|
|
1179
|
push @data, @{ $self->get_type_object->split($arg) }; |
|
644
|
|
|
|
|
2278
|
|
205
|
|
|
|
|
|
|
} |
206
|
|
|
|
|
|
|
} |
207
|
713
|
|
|
|
|
2986
|
my $missing = $self->get_missing; |
208
|
713
|
|
50
|
|
|
3096
|
my $position = $self->get_position || 1; |
209
|
713
|
|
|
|
|
2942
|
for ( 1 .. $position - 1 ) { |
210
|
0
|
|
|
|
|
0
|
unshift @data, $missing; |
211
|
|
|
|
|
|
|
} |
212
|
713
|
|
|
|
|
1399
|
my @char = @{ $self->get_entities }; # store old data for rollback |
|
713
|
|
|
|
|
2099
|
|
213
|
713
|
|
|
|
|
1841
|
eval { |
214
|
713
|
|
|
|
|
3254
|
$self->clear; |
215
|
713
|
|
|
|
|
3572
|
$self->insert(@data); |
216
|
|
|
|
|
|
|
}; |
217
|
713
|
100
|
|
|
|
2017
|
if ($@) { |
218
|
7
|
|
|
|
|
20
|
$self->clear; |
219
|
7
|
|
|
|
|
11
|
eval { $self->insert(@char) }; |
|
7
|
|
|
|
|
50
|
|
220
|
7
|
|
|
|
|
20
|
undef($@); |
221
|
7
|
|
|
|
|
19
|
throw 'InvalidData' => |
222
|
|
|
|
|
|
|
sprintf( 'Invalid data for row %s (type %s: %s)', |
223
|
|
|
|
|
|
|
$self->get_internal_name, $self->get_type, join( ' ', @data ) ); |
224
|
|
|
|
|
|
|
} |
225
|
706
|
|
|
|
|
3326
|
$self->set_annotations; |
226
|
706
|
|
|
|
|
8738
|
return $self; |
227
|
|
|
|
|
|
|
} |
228
|
|
|
|
|
|
|
|
229
|
|
|
|
|
|
|
=back |
230
|
|
|
|
|
|
|
|
231
|
|
|
|
|
|
|
=head2 ACCESSORS |
232
|
|
|
|
|
|
|
|
233
|
|
|
|
|
|
|
=over |
234
|
|
|
|
|
|
|
|
235
|
|
|
|
|
|
|
=item get_matrix() |
236
|
|
|
|
|
|
|
|
237
|
|
|
|
|
|
|
Gets the matrix (if any) this datum belongs to |
238
|
|
|
|
|
|
|
|
239
|
|
|
|
|
|
|
Type : Accessor |
240
|
|
|
|
|
|
|
Title : get_matrix |
241
|
|
|
|
|
|
|
Usage : my $matrix = $datum->get_matrix; |
242
|
|
|
|
|
|
|
Function: Retrieves the matrix the datum belongs to |
243
|
|
|
|
|
|
|
Returns : Bio::Phylo::Matrices::Matrix |
244
|
|
|
|
|
|
|
Args : NONE |
245
|
|
|
|
|
|
|
|
246
|
|
|
|
|
|
|
=cut |
247
|
|
|
|
|
|
|
|
248
|
133
|
|
|
133
|
1
|
327
|
sub get_matrix { shift->_get_container } |
249
|
|
|
|
|
|
|
|
250
|
|
|
|
|
|
|
=item get_char() |
251
|
|
|
|
|
|
|
|
252
|
|
|
|
|
|
|
Gets characters. |
253
|
|
|
|
|
|
|
|
254
|
|
|
|
|
|
|
Type : Accessor |
255
|
|
|
|
|
|
|
Title : get_char |
256
|
|
|
|
|
|
|
Usage : my $char = $datum->get_char; |
257
|
|
|
|
|
|
|
Function: Retrieves a datum's character value. |
258
|
|
|
|
|
|
|
Returns : In scalar context, returns a single |
259
|
|
|
|
|
|
|
character, or a string of characters |
260
|
|
|
|
|
|
|
(e.g. a DNA sequence, or a space |
261
|
|
|
|
|
|
|
delimited series of continuous characters). |
262
|
|
|
|
|
|
|
In list context, returns a list of characters |
263
|
|
|
|
|
|
|
(of zero or more characters). |
264
|
|
|
|
|
|
|
Args : NONE |
265
|
|
|
|
|
|
|
|
266
|
|
|
|
|
|
|
=cut |
267
|
|
|
|
|
|
|
|
268
|
|
|
|
|
|
|
sub get_char { |
269
|
1462
|
|
|
1462
|
1
|
5282
|
my $self = shift; |
270
|
1462
|
|
|
|
|
2198
|
my @data = @{ $self->get_entities }; |
|
1462
|
|
|
|
|
5574
|
|
271
|
1462
|
100
|
|
|
|
4487
|
if (@data) { |
272
|
715
|
100
|
|
|
|
22801
|
return wantarray ? @data : $self->get_type_object->join( \@data ); |
273
|
|
|
|
|
|
|
} |
274
|
|
|
|
|
|
|
else { |
275
|
747
|
50
|
|
|
|
3513
|
return wantarray ? () : ''; |
276
|
|
|
|
|
|
|
} |
277
|
|
|
|
|
|
|
} |
278
|
|
|
|
|
|
|
|
279
|
|
|
|
|
|
|
=item get_unaligned_char() |
280
|
|
|
|
|
|
|
|
281
|
|
|
|
|
|
|
Gets unaligned characters, i.e. without gap or missing symbols |
282
|
|
|
|
|
|
|
|
283
|
|
|
|
|
|
|
Type : Accessor |
284
|
|
|
|
|
|
|
Title : get_unaligned_char |
285
|
|
|
|
|
|
|
Usage : my $char = $datum->get_unaligned_char; |
286
|
|
|
|
|
|
|
Function: Retrieves a datum's unaligned character sequence |
287
|
|
|
|
|
|
|
Returns : In scalar context, returns a single |
288
|
|
|
|
|
|
|
character, or a string of characters |
289
|
|
|
|
|
|
|
(e.g. a DNA sequence, or a space |
290
|
|
|
|
|
|
|
delimited series of continuous characters). |
291
|
|
|
|
|
|
|
In list context, returns a list of characters |
292
|
|
|
|
|
|
|
(of zero or more characters). |
293
|
|
|
|
|
|
|
Args : NONE |
294
|
|
|
|
|
|
|
|
295
|
|
|
|
|
|
|
=cut |
296
|
|
|
|
|
|
|
|
297
|
|
|
|
|
|
|
sub get_unaligned_char { |
298
|
0
|
|
|
0
|
1
|
0
|
my $self = shift; |
299
|
0
|
|
|
|
|
0
|
my $gap = $self->get_gap; |
300
|
0
|
|
|
|
|
0
|
my $missing = $self->get_missing; |
301
|
0
|
|
|
|
|
0
|
my @char = $self->get_char; |
302
|
0
|
0
|
|
|
|
0
|
my @data = grep { $_ ne $gap && $_ ne $missing } @char; |
|
0
|
|
|
|
|
0
|
|
303
|
0
|
0
|
|
|
|
0
|
if (@data) { |
304
|
0
|
0
|
|
|
|
0
|
return wantarray ? @data : $self->get_type_object->join( \@data ); |
305
|
|
|
|
|
|
|
} |
306
|
|
|
|
|
|
|
else { |
307
|
0
|
0
|
|
|
|
0
|
return wantarray ? () : ''; |
308
|
|
|
|
|
|
|
} |
309
|
|
|
|
|
|
|
} |
310
|
|
|
|
|
|
|
|
311
|
|
|
|
|
|
|
=item get_length() |
312
|
|
|
|
|
|
|
|
313
|
|
|
|
|
|
|
Gets invocant number of characters. |
314
|
|
|
|
|
|
|
|
315
|
|
|
|
|
|
|
Type : Accessor |
316
|
|
|
|
|
|
|
Title : get_length |
317
|
|
|
|
|
|
|
Usage : my $length = $datum->get_length; |
318
|
|
|
|
|
|
|
Function: Retrieves a datum's length. |
319
|
|
|
|
|
|
|
Returns : a SCALAR integer. |
320
|
|
|
|
|
|
|
Args : NONE |
321
|
|
|
|
|
|
|
|
322
|
|
|
|
|
|
|
=cut |
323
|
|
|
|
|
|
|
|
324
|
|
|
|
|
|
|
sub get_length { |
325
|
623
|
|
|
623
|
1
|
1188
|
my $self = shift; |
326
|
623
|
100
|
|
|
|
2311
|
if ( my $matrix = $self->_get_container ) { |
327
|
39
|
|
|
|
|
92
|
return $matrix->get_nchar; |
328
|
|
|
|
|
|
|
} |
329
|
|
|
|
|
|
|
else { |
330
|
584
|
|
50
|
|
|
1091
|
return scalar( @{ $self->get_entities } ) + ( $self->get_position || 1 ) - 1; |
|
584
|
|
|
|
|
2018
|
|
331
|
|
|
|
|
|
|
} |
332
|
|
|
|
|
|
|
} |
333
|
|
|
|
|
|
|
|
334
|
|
|
|
|
|
|
=item get_by_index() |
335
|
|
|
|
|
|
|
|
336
|
|
|
|
|
|
|
Gets state at argument index. |
337
|
|
|
|
|
|
|
|
338
|
|
|
|
|
|
|
Type : Accessor |
339
|
|
|
|
|
|
|
Title : get_by_index |
340
|
|
|
|
|
|
|
Usage : my $val = $datum->get_by_index($i); |
341
|
|
|
|
|
|
|
Function: Retrieves state at index $i. |
342
|
|
|
|
|
|
|
Returns : a character state. |
343
|
|
|
|
|
|
|
Args : INT |
344
|
|
|
|
|
|
|
|
345
|
|
|
|
|
|
|
=cut |
346
|
|
|
|
|
|
|
|
347
|
|
|
|
|
|
|
sub get_by_index { |
348
|
50
|
|
|
50
|
1
|
71
|
my ( $self, $index ) = @_; |
349
|
50
|
|
|
|
|
108
|
$logger->debug($index); |
350
|
50
|
|
50
|
|
|
87
|
my $offset = ( $self->get_position || 1 ) - 1; |
351
|
50
|
50
|
|
|
|
89
|
return $self->get_type_object->get_missing if $offset > $index; |
352
|
50
|
|
|
|
|
116
|
my $val = $self->SUPER::get_by_index( $index - $offset ); |
353
|
50
|
50
|
|
|
|
112
|
return defined $val ? $val : $self->get_type_object->get_missing; |
354
|
|
|
|
|
|
|
} |
355
|
|
|
|
|
|
|
|
356
|
|
|
|
|
|
|
=item get_index_of() |
357
|
|
|
|
|
|
|
|
358
|
|
|
|
|
|
|
Returns the index of the first occurrence of the |
359
|
|
|
|
|
|
|
state observation in the datum or undef if the datum |
360
|
|
|
|
|
|
|
doesn't contain the argument |
361
|
|
|
|
|
|
|
|
362
|
|
|
|
|
|
|
Type : Generic query |
363
|
|
|
|
|
|
|
Title : get_index_of |
364
|
|
|
|
|
|
|
Usage : my $i = $datum->get_index_of($state) |
365
|
|
|
|
|
|
|
Function: Returns the index of the first occurrence of the |
366
|
|
|
|
|
|
|
state observation in the datum or undef if the datum |
367
|
|
|
|
|
|
|
doesn't contain the argument |
368
|
|
|
|
|
|
|
Returns : An index or undef |
369
|
|
|
|
|
|
|
Args : A contained object |
370
|
|
|
|
|
|
|
|
371
|
|
|
|
|
|
|
=cut |
372
|
|
|
|
|
|
|
|
373
|
|
|
|
|
|
|
sub get_index_of { |
374
|
0
|
|
|
0
|
1
|
0
|
my ( $self, $obj ) = @_; |
375
|
0
|
|
|
|
|
0
|
my $is_numerical = |
376
|
|
|
|
|
|
|
$self->get_type =~ m/^(Continuous|Standard|Restriction)$/; |
377
|
0
|
|
|
|
|
0
|
my $i = 0; |
378
|
0
|
|
|
|
|
0
|
for my $ent ( @{ $self->get_entities } ) { |
|
0
|
|
|
|
|
0
|
|
379
|
0
|
0
|
|
|
|
0
|
if ($is_numerical) { |
380
|
0
|
0
|
|
|
|
0
|
return $i if $obj == $ent; |
381
|
|
|
|
|
|
|
} |
382
|
|
|
|
|
|
|
else { |
383
|
0
|
0
|
|
|
|
0
|
return $i if $obj eq $ent; |
384
|
|
|
|
|
|
|
} |
385
|
0
|
|
|
|
|
0
|
$i++; |
386
|
|
|
|
|
|
|
} |
387
|
0
|
|
|
|
|
0
|
return; |
388
|
|
|
|
|
|
|
} |
389
|
|
|
|
|
|
|
|
390
|
|
|
|
|
|
|
=back |
391
|
|
|
|
|
|
|
|
392
|
|
|
|
|
|
|
=head2 TESTS |
393
|
|
|
|
|
|
|
|
394
|
|
|
|
|
|
|
=over |
395
|
|
|
|
|
|
|
|
396
|
|
|
|
|
|
|
=item can_contain() |
397
|
|
|
|
|
|
|
|
398
|
|
|
|
|
|
|
Tests if invocant can contain argument. |
399
|
|
|
|
|
|
|
|
400
|
|
|
|
|
|
|
Type : Test |
401
|
|
|
|
|
|
|
Title : can_contain |
402
|
|
|
|
|
|
|
Usage : &do_something if $datum->can_contain( @args ); |
403
|
|
|
|
|
|
|
Function: Tests if $datum can contain @args |
404
|
|
|
|
|
|
|
Returns : BOOLEAN |
405
|
|
|
|
|
|
|
Args : One or more arguments as can be provided to set_char |
406
|
|
|
|
|
|
|
|
407
|
|
|
|
|
|
|
=cut |
408
|
|
|
|
|
|
|
|
409
|
|
|
|
|
|
|
sub can_contain { |
410
|
719
|
|
|
719
|
1
|
1515
|
my $self = shift; |
411
|
719
|
|
|
|
|
17613
|
my @data = @_; |
412
|
719
|
50
|
|
|
|
2240
|
if ( my $obj = $self->get_type_object ) { |
413
|
719
|
50
|
|
|
|
6307
|
if ( $obj->isa('Bio::Phylo::Matrices::Datatype::Mixed') ) { |
414
|
0
|
|
|
|
|
0
|
my @split; |
415
|
0
|
|
|
|
|
0
|
for my $datum (@data) { |
416
|
0
|
0
|
|
|
|
0
|
if ( looks_like_implementor( $datum, 'get_char' ) ) { |
|
|
0
|
|
|
|
|
|
417
|
0
|
|
|
|
|
0
|
my @tmp = $datum->get_char(); |
418
|
0
|
|
|
|
|
0
|
my $i = $datum->get_position() - 1; |
419
|
0
|
|
|
|
|
0
|
for (@tmp) { |
420
|
0
|
|
|
|
|
0
|
$split[ $i++ ] = $_; |
421
|
|
|
|
|
|
|
} |
422
|
|
|
|
|
|
|
} |
423
|
|
|
|
|
|
|
elsif ( looks_like_instance( $datum, 'ARRAY' ) ) { |
424
|
0
|
|
|
|
|
0
|
push @split, @{$datum}; |
|
0
|
|
|
|
|
0
|
|
425
|
|
|
|
|
|
|
} |
426
|
|
|
|
|
|
|
else { |
427
|
0
|
|
|
|
|
0
|
my $subtype = $obj->get_type_for_site( scalar(@split) ); |
428
|
0
|
|
|
|
|
0
|
push @split, @{ $subtype->split($datum) }; |
|
0
|
|
|
|
|
0
|
|
429
|
|
|
|
|
|
|
} |
430
|
|
|
|
|
|
|
} |
431
|
|
|
|
|
|
|
|
432
|
|
|
|
|
|
|
#return 1; |
433
|
0
|
|
|
|
|
0
|
for my $i ( 1 .. scalar(@split) ) { |
434
|
0
|
|
|
|
|
0
|
my $subtype = $obj->get_type_for_site( $i - 1 ); |
435
|
0
|
0
|
|
|
|
0
|
next if $subtype->is_valid( $split[ $i - 1 ] ); |
436
|
0
|
|
|
|
|
0
|
throw 'InvalidData' => sprintf( |
437
|
|
|
|
|
|
|
'Invalid char %s at pos %s for type %s', |
438
|
|
|
|
|
|
|
$split[ $i - 1 ], |
439
|
|
|
|
|
|
|
$i, $subtype->get_type, |
440
|
|
|
|
|
|
|
); |
441
|
|
|
|
|
|
|
} |
442
|
0
|
|
|
|
|
0
|
return 1; |
443
|
|
|
|
|
|
|
} |
444
|
|
|
|
|
|
|
else { |
445
|
719
|
|
|
|
|
2888
|
return $obj->is_valid(@data); |
446
|
|
|
|
|
|
|
} |
447
|
|
|
|
|
|
|
} |
448
|
|
|
|
|
|
|
else { |
449
|
0
|
|
|
|
|
0
|
throw 'API' => "No associated type object found,\n" |
450
|
|
|
|
|
|
|
. "this is a bug - please report - thanks"; |
451
|
|
|
|
|
|
|
} |
452
|
|
|
|
|
|
|
} |
453
|
|
|
|
|
|
|
|
454
|
|
|
|
|
|
|
=back |
455
|
|
|
|
|
|
|
|
456
|
|
|
|
|
|
|
=head2 CALCULATIONS |
457
|
|
|
|
|
|
|
|
458
|
|
|
|
|
|
|
=over |
459
|
|
|
|
|
|
|
|
460
|
|
|
|
|
|
|
=item calc_state_counts() |
461
|
|
|
|
|
|
|
|
462
|
|
|
|
|
|
|
Calculates occurrences of states. |
463
|
|
|
|
|
|
|
|
464
|
|
|
|
|
|
|
Type : Calculation |
465
|
|
|
|
|
|
|
Title : calc_state_counts |
466
|
|
|
|
|
|
|
Usage : my %counts = %{ $datum->calc_state_counts }; |
467
|
|
|
|
|
|
|
Function: Calculates occurrences of states. |
468
|
|
|
|
|
|
|
Returns : Hashref: keys are states, values are counts |
469
|
|
|
|
|
|
|
Args : Optional - one or more states to focus on |
470
|
|
|
|
|
|
|
|
471
|
|
|
|
|
|
|
=cut |
472
|
|
|
|
|
|
|
|
473
|
|
|
|
|
|
|
sub calc_state_counts { |
474
|
23
|
|
|
23
|
1
|
30
|
my $self = shift; |
475
|
|
|
|
|
|
|
|
476
|
|
|
|
|
|
|
# maybe there should be an option to bin continuous values |
477
|
|
|
|
|
|
|
# in X categories, and return the frequencies of those? Anyway, |
478
|
|
|
|
|
|
|
# Hennig86 seems to want continuous values to be counted as well, |
479
|
|
|
|
|
|
|
# so not throwing an exception here. |
480
|
|
|
|
|
|
|
#if ( $self->get_type =~ /^continuous$/i ) { |
481
|
|
|
|
|
|
|
# throw 'BadArgs' => 'Matrix holds continuous values'; |
482
|
|
|
|
|
|
|
#} |
483
|
23
|
|
|
|
|
28
|
my %counts; |
484
|
23
|
50
|
|
|
|
41
|
if (@_) { |
485
|
0
|
|
|
|
|
0
|
my %focus = map { $_ => 1 } @_; |
|
0
|
|
|
|
|
0
|
|
486
|
0
|
|
|
|
|
0
|
my @char = $self->get_char; |
487
|
0
|
|
|
|
|
0
|
for my $c (@char) { |
488
|
0
|
0
|
|
|
|
0
|
if ( exists $focus{$c} ) { |
489
|
0
|
0
|
|
|
|
0
|
if ( not exists $counts{$c} ) { |
490
|
0
|
|
|
|
|
0
|
$counts{$c} = 1; |
491
|
|
|
|
|
|
|
} |
492
|
|
|
|
|
|
|
else { |
493
|
0
|
|
|
|
|
0
|
$counts{$c}++; |
494
|
|
|
|
|
|
|
} |
495
|
|
|
|
|
|
|
} |
496
|
|
|
|
|
|
|
} |
497
|
|
|
|
|
|
|
} |
498
|
|
|
|
|
|
|
else { |
499
|
23
|
|
|
|
|
35
|
my @char = $self->get_char; |
500
|
23
|
|
|
|
|
36
|
for my $c (@char) { |
501
|
101
|
100
|
|
|
|
148
|
if ( not exists $counts{$c} ) { |
502
|
80
|
|
|
|
|
115
|
$counts{$c} = 1; |
503
|
|
|
|
|
|
|
} |
504
|
|
|
|
|
|
|
else { |
505
|
21
|
|
|
|
|
29
|
$counts{$c}++; |
506
|
|
|
|
|
|
|
} |
507
|
|
|
|
|
|
|
} |
508
|
|
|
|
|
|
|
} |
509
|
23
|
|
|
|
|
47
|
return \%counts; |
510
|
|
|
|
|
|
|
} |
511
|
|
|
|
|
|
|
|
512
|
|
|
|
|
|
|
=item calc_distance() |
513
|
|
|
|
|
|
|
|
514
|
|
|
|
|
|
|
Calculates the distance between the invocant and argument |
515
|
|
|
|
|
|
|
|
516
|
|
|
|
|
|
|
Type : Calculation |
517
|
|
|
|
|
|
|
Title : calc_distance |
518
|
|
|
|
|
|
|
Usage : my $dist = $datum1->calc_distance($datum2); |
519
|
|
|
|
|
|
|
Function: Calculates pairwise distance |
520
|
|
|
|
|
|
|
Returns : A number, the distance per site |
521
|
|
|
|
|
|
|
Args : Another datum to calculate the distance to |
522
|
|
|
|
|
|
|
Comments: Assumes the sequences are aligned. Calculates |
523
|
|
|
|
|
|
|
substitutions / total non-missing and non-gapped sites. |
524
|
|
|
|
|
|
|
=cut |
525
|
|
|
|
|
|
|
|
526
|
|
|
|
|
|
|
sub calc_distance { |
527
|
0
|
|
|
0
|
1
|
0
|
my ( $self, $other ) = @_; |
528
|
0
|
|
|
|
|
0
|
my @c1 = $self->get_char; |
529
|
0
|
|
|
|
|
0
|
my @c2 = $other->get_char; |
530
|
0
|
|
|
|
|
0
|
my $t = $self->get_type_object; |
531
|
0
|
|
|
|
|
0
|
my $m = $t->get_missing; |
532
|
0
|
|
|
|
|
0
|
my $g = $t->get_gap; |
533
|
0
|
|
|
|
|
0
|
my $subst = 0; |
534
|
0
|
|
|
|
|
0
|
my $total = 0; |
535
|
0
|
|
|
|
|
0
|
for my $i ( 0 .. $#c1 ) { |
536
|
0
|
0
|
0
|
|
|
0
|
next if $c1[$i] eq $m or $c1[$i] eq $g or $c2[$i] eq $m or $c2[$i] eq $g; |
|
|
|
0
|
|
|
|
|
|
|
|
0
|
|
|
|
|
537
|
0
|
|
|
|
|
0
|
$subst += $c1[$i] ne $c2[$i]; |
538
|
0
|
|
|
|
|
0
|
$total++; |
539
|
|
|
|
|
|
|
} |
540
|
0
|
0
|
|
|
|
0
|
return $total ? $subst / $total : 9**9**9; |
541
|
|
|
|
|
|
|
} |
542
|
|
|
|
|
|
|
|
543
|
|
|
|
|
|
|
=item calc_state_frequencies() |
544
|
|
|
|
|
|
|
|
545
|
|
|
|
|
|
|
Calculates the frequencies of the states observed in the matrix. |
546
|
|
|
|
|
|
|
|
547
|
|
|
|
|
|
|
Type : Calculation |
548
|
|
|
|
|
|
|
Title : calc_state_frequencies |
549
|
|
|
|
|
|
|
Usage : my %freq = %{ $object->calc_state_frequencies() }; |
550
|
|
|
|
|
|
|
Function: Calculates state frequencies |
551
|
|
|
|
|
|
|
Returns : A hash, keys are state symbols, values are frequencies |
552
|
|
|
|
|
|
|
Args : Optional: |
553
|
|
|
|
|
|
|
# if true, counts missing (usually the '?' symbol) as a state |
554
|
|
|
|
|
|
|
# in the final tallies. Otherwise, missing states are ignored |
555
|
|
|
|
|
|
|
-missing => 1 |
556
|
|
|
|
|
|
|
# if true, counts gaps (usually the '-' symbol) as a state |
557
|
|
|
|
|
|
|
# in the final tallies. Otherwise, gap states are ignored |
558
|
|
|
|
|
|
|
-gap => 1 |
559
|
|
|
|
|
|
|
Comments: Throws exception if matrix holds continuous values |
560
|
|
|
|
|
|
|
|
561
|
|
|
|
|
|
|
=cut |
562
|
|
|
|
|
|
|
|
563
|
|
|
|
|
|
|
sub calc_state_frequencies { |
564
|
5
|
|
|
5
|
1
|
9
|
my $self = shift; |
565
|
5
|
|
|
|
|
18
|
my $counts = $self->calc_state_counts; |
566
|
5
|
|
|
|
|
13
|
my %args = looks_like_hash @_; |
567
|
5
|
|
|
|
|
8
|
for my $arg (qw(missing gap)) { |
568
|
10
|
50
|
|
|
|
27
|
if ( not exists $args{"-${arg}"} ) { |
569
|
10
|
|
|
|
|
15
|
my $method = "get_${arg}"; |
570
|
10
|
|
|
|
|
29
|
my $symbol = $self->$method; |
571
|
10
|
|
|
|
|
20
|
delete $counts->{$symbol}; |
572
|
|
|
|
|
|
|
} |
573
|
|
|
|
|
|
|
} |
574
|
5
|
|
|
|
|
7
|
my $total = 0; |
575
|
5
|
|
|
|
|
7
|
$total += $_ for values %{$counts}; |
|
5
|
|
|
|
|
18
|
|
576
|
5
|
50
|
|
|
|
12
|
if ( $total > 0 ) { |
577
|
5
|
|
|
|
|
6
|
for my $state ( keys %{$counts} ) { |
|
5
|
|
|
|
|
11
|
|
578
|
20
|
|
|
|
|
30
|
$counts->{$state} /= $total; |
579
|
|
|
|
|
|
|
} |
580
|
|
|
|
|
|
|
} |
581
|
5
|
|
|
|
|
12
|
return $counts; |
582
|
|
|
|
|
|
|
} |
583
|
|
|
|
|
|
|
|
584
|
|
|
|
|
|
|
=back |
585
|
|
|
|
|
|
|
|
586
|
|
|
|
|
|
|
=head2 METHODS |
587
|
|
|
|
|
|
|
|
588
|
|
|
|
|
|
|
=over |
589
|
|
|
|
|
|
|
|
590
|
|
|
|
|
|
|
=item reverse() |
591
|
|
|
|
|
|
|
|
592
|
|
|
|
|
|
|
Reverses contents. |
593
|
|
|
|
|
|
|
|
594
|
|
|
|
|
|
|
Type : Method |
595
|
|
|
|
|
|
|
Title : reverse |
596
|
|
|
|
|
|
|
Usage : $datum->reverse; |
597
|
|
|
|
|
|
|
Function: Reverses a datum's contained characters |
598
|
|
|
|
|
|
|
Returns : Returns modified $datum |
599
|
|
|
|
|
|
|
Args : NONE |
600
|
|
|
|
|
|
|
|
601
|
|
|
|
|
|
|
=cut |
602
|
|
|
|
|
|
|
|
603
|
|
|
|
|
|
|
sub reverse { |
604
|
0
|
|
|
0
|
1
|
0
|
my $self = shift; |
605
|
0
|
|
|
|
|
0
|
my @char = $self->get_char; |
606
|
0
|
|
|
|
|
0
|
my @reversed = reverse(@char); |
607
|
0
|
|
|
|
|
0
|
$self->set_char( \@reversed ); |
608
|
|
|
|
|
|
|
} |
609
|
|
|
|
|
|
|
|
610
|
|
|
|
|
|
|
=item concat() |
611
|
|
|
|
|
|
|
|
612
|
|
|
|
|
|
|
Appends argument to invocant. |
613
|
|
|
|
|
|
|
|
614
|
|
|
|
|
|
|
Type : Method |
615
|
|
|
|
|
|
|
Title : reverse |
616
|
|
|
|
|
|
|
Usage : $datum->concat($datum1); |
617
|
|
|
|
|
|
|
Function: Appends $datum1 to $datum |
618
|
|
|
|
|
|
|
Returns : Returns modified $datum |
619
|
|
|
|
|
|
|
Args : NONE |
620
|
|
|
|
|
|
|
|
621
|
|
|
|
|
|
|
=cut |
622
|
|
|
|
|
|
|
|
623
|
|
|
|
|
|
|
sub concat { |
624
|
0
|
|
|
0
|
1
|
0
|
my ( $self, @data ) = @_; |
625
|
0
|
|
|
|
|
0
|
$logger->info("concatenating objects"); |
626
|
0
|
|
|
|
|
0
|
my @newchars; |
627
|
0
|
|
|
|
|
0
|
my @self_chars = $self->get_char; |
628
|
0
|
|
|
|
|
0
|
my $self_i = $self->get_position - 1; |
629
|
0
|
|
|
|
|
0
|
my $self_j = $self->get_length - 1 + $self_i; |
630
|
0
|
|
|
|
|
0
|
@newchars[ $self_i .. $self_j ] = @self_chars; |
631
|
0
|
|
|
|
|
0
|
for my $datum (@data) { |
632
|
0
|
|
|
|
|
0
|
my @chars = $datum->get_char; |
633
|
0
|
|
|
|
|
0
|
my $i = $datum->get_position - 1; |
634
|
0
|
|
|
|
|
0
|
my $j = $datum->get_length - 1 + $i; |
635
|
0
|
|
|
|
|
0
|
@newchars[ $i .. $j ] = @chars; |
636
|
|
|
|
|
|
|
} |
637
|
0
|
|
|
|
|
0
|
my $missing = $self->get_missing; |
638
|
0
|
|
|
|
|
0
|
for my $i ( 0 .. $#newchars ) { |
639
|
0
|
0
|
|
|
|
0
|
$newchars[$i] = $missing if !defined $newchars[$i]; |
640
|
|
|
|
|
|
|
} |
641
|
0
|
|
|
|
|
0
|
$self->set_char( \@newchars ); |
642
|
|
|
|
|
|
|
} |
643
|
|
|
|
|
|
|
|
644
|
|
|
|
|
|
|
=item consense() |
645
|
|
|
|
|
|
|
|
646
|
|
|
|
|
|
|
Creates consensus sequence out of arguments |
647
|
|
|
|
|
|
|
|
648
|
|
|
|
|
|
|
Type : Method |
649
|
|
|
|
|
|
|
Title : consense |
650
|
|
|
|
|
|
|
Usage : my @chars = $datum->consense($datum1,...); |
651
|
|
|
|
|
|
|
Function: Creates consensus sequence out of arguments |
652
|
|
|
|
|
|
|
Returns : Returns @chars or $seq |
653
|
|
|
|
|
|
|
Args : NONE |
654
|
|
|
|
|
|
|
|
655
|
|
|
|
|
|
|
=cut |
656
|
|
|
|
|
|
|
|
657
|
|
|
|
|
|
|
sub consense { |
658
|
0
|
|
|
0
|
1
|
0
|
my @data = @_; |
659
|
|
|
|
|
|
|
|
660
|
|
|
|
|
|
|
# build two-dimensional array of character states |
661
|
0
|
|
|
|
|
0
|
my @chars; |
662
|
0
|
|
|
|
|
0
|
for my $datum ( @data ) { |
663
|
0
|
|
|
|
|
0
|
my @char = $datum->get_char; |
664
|
0
|
|
|
|
|
0
|
push @chars, \@char; |
665
|
|
|
|
|
|
|
} |
666
|
|
|
|
|
|
|
|
667
|
|
|
|
|
|
|
# get special symbols |
668
|
0
|
|
|
|
|
0
|
my $length = $data[0]->get_length; |
669
|
0
|
|
|
|
|
0
|
my $to = $data[0]->get_type_object; |
670
|
0
|
|
|
|
|
0
|
my $m = $to->get_missing; |
671
|
0
|
|
|
|
|
0
|
my $g = $to->get_gap; |
672
|
|
|
|
|
|
|
|
673
|
|
|
|
|
|
|
# build result |
674
|
0
|
|
|
|
|
0
|
my @result; |
675
|
0
|
|
|
|
|
0
|
for my $i ( 0 .. ( $length - 1 ) ) { |
676
|
0
|
|
|
|
|
0
|
my %col; |
677
|
|
|
|
|
|
|
|
678
|
|
|
|
|
|
|
# get distinct states for column, ignore missing and gap |
679
|
0
|
|
|
|
|
0
|
ROW: for my $row ( @chars ) { |
680
|
0
|
|
|
|
|
0
|
my $c = $row->[$i]; |
681
|
0
|
0
|
0
|
|
|
0
|
next ROW if $c eq $m or $c eq $g; |
682
|
0
|
|
|
|
|
0
|
$col{$c} = 1; |
683
|
|
|
|
|
|
|
} |
684
|
|
|
|
|
|
|
|
685
|
|
|
|
|
|
|
# get ambiguity symbol or missing |
686
|
0
|
|
|
|
|
0
|
my @states = keys %col; |
687
|
0
|
0
|
|
|
|
0
|
if ( @states ) { |
688
|
0
|
|
0
|
|
|
0
|
push @result, $to->get_symbol_for_states(@states) || $m; |
689
|
|
|
|
|
|
|
} |
690
|
|
|
|
|
|
|
else { |
691
|
0
|
|
|
|
|
0
|
push @result, $m; |
692
|
|
|
|
|
|
|
} |
693
|
|
|
|
|
|
|
} |
694
|
|
|
|
|
|
|
|
695
|
|
|
|
|
|
|
# return result |
696
|
0
|
0
|
|
|
|
0
|
return wantarray ? @result : $to->join(@result); |
697
|
|
|
|
|
|
|
} |
698
|
|
|
|
|
|
|
|
699
|
|
|
|
|
|
|
=begin comment |
700
|
|
|
|
|
|
|
|
701
|
|
|
|
|
|
|
Validates invocant data contents. |
702
|
|
|
|
|
|
|
|
703
|
|
|
|
|
|
|
Type : Method |
704
|
|
|
|
|
|
|
Title : validate |
705
|
|
|
|
|
|
|
Usage : $datum->validate; |
706
|
|
|
|
|
|
|
Function: Validates character data contained by $datum |
707
|
|
|
|
|
|
|
Returns : True or throws Bio::Phylo::Util::Exceptions::InvalidData |
708
|
|
|
|
|
|
|
Args : NONE |
709
|
|
|
|
|
|
|
|
710
|
|
|
|
|
|
|
=end comment |
711
|
|
|
|
|
|
|
|
712
|
|
|
|
|
|
|
=cut |
713
|
|
|
|
|
|
|
|
714
|
|
|
|
|
|
|
sub _validate { |
715
|
752
|
|
|
752
|
|
1663
|
my $self = shift; |
716
|
752
|
100
|
|
|
|
2317
|
if ( !$self->get_type_object->is_valid($self) ) { |
717
|
3
|
|
|
|
|
8
|
throw 'InvalidData' => 'Invalid data!'; |
718
|
|
|
|
|
|
|
} |
719
|
|
|
|
|
|
|
} |
720
|
|
|
|
|
|
|
|
721
|
|
|
|
|
|
|
=item to_xml() |
722
|
|
|
|
|
|
|
|
723
|
|
|
|
|
|
|
Serializes datum to nexml format. |
724
|
|
|
|
|
|
|
|
725
|
|
|
|
|
|
|
Type : Format convertor |
726
|
|
|
|
|
|
|
Title : to_xml |
727
|
|
|
|
|
|
|
Usage : my $xml = $datum->to_xml; |
728
|
|
|
|
|
|
|
Function: Converts datum object into a nexml element structure. |
729
|
|
|
|
|
|
|
Returns : Nexml block (SCALAR). |
730
|
|
|
|
|
|
|
Args : -chars => [] # optional, an array ref of character IDs |
731
|
|
|
|
|
|
|
-states => {} # optional, a hash ref of state IDs |
732
|
|
|
|
|
|
|
-symbols => {} # optional, a hash ref of symbols |
733
|
|
|
|
|
|
|
-special => {} # optional, a hash ref of special symbol IDs |
734
|
|
|
|
|
|
|
|
735
|
|
|
|
|
|
|
=cut |
736
|
|
|
|
|
|
|
|
737
|
|
|
|
|
|
|
sub to_xml { |
738
|
0
|
|
|
0
|
1
|
0
|
my $self = shift; |
739
|
0
|
|
|
|
|
0
|
my %args = looks_like_hash @_; |
740
|
0
|
|
|
|
|
0
|
my $char_ids = $args{'-chars'}; |
741
|
0
|
|
|
|
|
0
|
my $state_ids = $args{'-states'}; |
742
|
0
|
|
|
|
|
0
|
my $special = $args{'-special'}; |
743
|
0
|
0
|
|
|
|
0
|
if ( my $taxon = $self->get_taxon ) { |
744
|
0
|
|
|
|
|
0
|
$self->set_attributes( 'otu' => $taxon->get_xml_id ); |
745
|
|
|
|
|
|
|
} |
746
|
0
|
|
|
|
|
0
|
my @char = $self->get_char; |
747
|
0
|
|
|
|
|
0
|
my ( $missing, $gap ) = ( $self->get_missing, $self->get_gap ); |
748
|
0
|
|
|
|
|
0
|
my $xml = $self->get_xml_tag; |
749
|
0
|
0
|
|
|
|
0
|
if ( not $args{'-compact'} ) { |
750
|
0
|
|
|
|
|
0
|
for my $i ( 0 .. $#char ) { |
751
|
0
|
|
|
|
|
0
|
my ( $c, $s ); |
752
|
0
|
0
|
0
|
|
|
0
|
if ( $missing ne $char[$i] and $gap ne $char[$i] ) { |
|
|
0
|
0
|
|
|
|
|
753
|
0
|
0
|
0
|
|
|
0
|
if ( $char_ids and $char_ids->[$i] ) { |
754
|
0
|
|
|
|
|
0
|
$c = $char_ids->[$i]; |
755
|
|
|
|
|
|
|
} |
756
|
|
|
|
|
|
|
else { |
757
|
0
|
|
|
|
|
0
|
$c = $i; |
758
|
|
|
|
|
|
|
} |
759
|
0
|
0
|
0
|
|
|
0
|
if ( $state_ids and $state_ids->{ uc $char[$i] } ) { |
760
|
0
|
|
|
|
|
0
|
$s = $state_ids->{ uc $char[$i] }; |
761
|
|
|
|
|
|
|
} |
762
|
|
|
|
|
|
|
else { |
763
|
0
|
|
|
|
|
0
|
$s = uc $char[$i]; |
764
|
|
|
|
|
|
|
} |
765
|
|
|
|
|
|
|
} |
766
|
|
|
|
|
|
|
elsif ( $missing eq $char[$i] or $gap eq $char[$i] ) { |
767
|
0
|
0
|
0
|
|
|
0
|
if ( $char_ids and $char_ids->[$i] ) { |
768
|
0
|
|
|
|
|
0
|
$c = $char_ids->[$i]; |
769
|
|
|
|
|
|
|
} |
770
|
|
|
|
|
|
|
else { |
771
|
0
|
|
|
|
|
0
|
$c = $i; |
772
|
|
|
|
|
|
|
} |
773
|
0
|
0
|
0
|
|
|
0
|
if ( $special and $special->{ $char[$i] } ) { |
774
|
0
|
|
|
|
|
0
|
$s = $special->{ $char[$i] }; |
775
|
|
|
|
|
|
|
} |
776
|
|
|
|
|
|
|
else { |
777
|
0
|
|
|
|
|
0
|
$s = $char[$i]; |
778
|
|
|
|
|
|
|
} |
779
|
|
|
|
|
|
|
} |
780
|
|
|
|
|
|
|
|
781
|
|
|
|
|
|
|
# $cell->set_attributes( 'char' => $c, 'state' => $s ); |
782
|
|
|
|
|
|
|
# $xml .= $cell->get_xml_tag(1); |
783
|
0
|
|
|
|
|
0
|
$xml .= sprintf( '<cell char="%s" state="%s"/>', $c, $s ); |
784
|
|
|
|
|
|
|
} |
785
|
|
|
|
|
|
|
} |
786
|
|
|
|
|
|
|
else { |
787
|
0
|
|
|
|
|
0
|
my @tmp = map { uc $_ } @char; |
|
0
|
|
|
|
|
0
|
|
788
|
0
|
|
|
|
|
0
|
my $seq = Bio::Phylo::NeXML::Writable->new( |
789
|
|
|
|
|
|
|
'-tag' => 'seq', |
790
|
|
|
|
|
|
|
'-identifiable' => 0, |
791
|
|
|
|
|
|
|
); |
792
|
0
|
|
|
|
|
0
|
my $seq_text = $self->get_type_object->join( \@tmp ); |
793
|
0
|
|
|
|
|
0
|
$xml .= |
794
|
|
|
|
|
|
|
$seq->get_xml_tag . "\n$seq_text\n" . "</" . $seq->get_tag . ">"; |
795
|
|
|
|
|
|
|
} |
796
|
0
|
|
|
|
|
0
|
$xml .= sprintf( '</%s>', $self->get_tag ); |
797
|
0
|
|
|
|
|
0
|
return $xml; |
798
|
|
|
|
|
|
|
} |
799
|
|
|
|
|
|
|
|
800
|
|
|
|
|
|
|
=item to_dom() |
801
|
|
|
|
|
|
|
|
802
|
|
|
|
|
|
|
Analog to to_xml. |
803
|
|
|
|
|
|
|
|
804
|
|
|
|
|
|
|
Type : Serializer |
805
|
|
|
|
|
|
|
Title : to_dom |
806
|
|
|
|
|
|
|
Usage : $datum->to_dom |
807
|
|
|
|
|
|
|
Function: Generates a DOM subtree from the invocant |
808
|
|
|
|
|
|
|
and its contained objects |
809
|
|
|
|
|
|
|
Returns : an XML::LibXML::Element object |
810
|
|
|
|
|
|
|
Args : none |
811
|
|
|
|
|
|
|
|
812
|
|
|
|
|
|
|
=cut |
813
|
|
|
|
|
|
|
|
814
|
|
|
|
|
|
|
sub to_dom { |
815
|
0
|
|
|
0
|
1
|
0
|
my $self = shift; |
816
|
0
|
|
|
|
|
0
|
my $dom = $_[0]; |
817
|
0
|
|
|
|
|
0
|
my @args = @_; |
818
|
|
|
|
|
|
|
|
819
|
|
|
|
|
|
|
# handle dom factory object... |
820
|
0
|
0
|
0
|
|
|
0
|
if ( looks_like_instance( $dom, 'SCALAR' ) |
821
|
|
|
|
|
|
|
&& $dom->_type == _DOMCREATOR_ ) |
822
|
|
|
|
|
|
|
{ |
823
|
0
|
|
|
|
|
0
|
splice( @args, 0, 1 ); |
824
|
|
|
|
|
|
|
} |
825
|
|
|
|
|
|
|
else { |
826
|
0
|
|
|
|
|
0
|
$dom = $Bio::Phylo::NeXML::DOM::DOM; |
827
|
0
|
0
|
|
|
|
0
|
unless ($dom) { |
828
|
0
|
|
|
|
|
0
|
throw 'BadArgs' => 'DOM factory object not provided'; |
829
|
|
|
|
|
|
|
} |
830
|
|
|
|
|
|
|
} |
831
|
|
|
|
|
|
|
##### make sure argument handling works here.... |
832
|
0
|
|
|
|
|
0
|
my %args = looks_like_hash @args; |
833
|
0
|
|
|
|
|
0
|
my $char_ids = $args{'-chars'}; |
834
|
0
|
|
|
|
|
0
|
my $state_ids = $args{'-states'}; |
835
|
0
|
|
|
|
|
0
|
my $special = $args{'-special'}; |
836
|
0
|
0
|
|
|
|
0
|
if ( my $taxon = $self->get_taxon ) { |
837
|
0
|
|
|
|
|
0
|
$self->set_attributes( 'otu' => $taxon->get_xml_id ); |
838
|
|
|
|
|
|
|
} |
839
|
0
|
|
|
|
|
0
|
my @char = $self->get_char; |
840
|
0
|
|
|
|
|
0
|
my ( $missing, $gap ) = ( $self->get_missing, $self->get_gap ); |
841
|
0
|
|
|
|
|
0
|
my $elt = $self->get_dom_elt($dom); |
842
|
0
|
0
|
|
|
|
0
|
if ( not $args{'-compact'} ) { |
843
|
0
|
|
|
|
|
0
|
for my $i ( 0 .. $#char ) { |
844
|
0
|
0
|
0
|
|
|
0
|
if ( $missing ne $char[$i] and $gap ne $char[$i] ) { |
|
|
0
|
0
|
|
|
|
|
845
|
0
|
|
|
|
|
0
|
my ( $c, $s ); |
846
|
0
|
0
|
0
|
|
|
0
|
if ( $char_ids and $char_ids->[$i] ) { |
847
|
0
|
|
|
|
|
0
|
$c = $char_ids->[$i]; |
848
|
|
|
|
|
|
|
} |
849
|
|
|
|
|
|
|
else { |
850
|
0
|
|
|
|
|
0
|
$c = $i; |
851
|
|
|
|
|
|
|
} |
852
|
0
|
0
|
0
|
|
|
0
|
if ( $state_ids and $state_ids->{ uc $char[$i] } ) { |
853
|
0
|
|
|
|
|
0
|
$s = $state_ids->{ uc $char[$i] }; |
854
|
|
|
|
|
|
|
} |
855
|
|
|
|
|
|
|
else { |
856
|
0
|
|
|
|
|
0
|
$s = uc $char[$i]; |
857
|
|
|
|
|
|
|
} |
858
|
0
|
|
|
|
|
0
|
my $cell_elt = $dom->create_element( '-tag' => 'cell' ); |
859
|
0
|
|
|
|
|
0
|
$cell_elt->set_attributes( 'char' => $c ); |
860
|
0
|
|
|
|
|
0
|
$cell_elt->set_attributes( 'state' => $s ); |
861
|
0
|
|
|
|
|
0
|
$elt->set_child($cell_elt); |
862
|
|
|
|
|
|
|
} |
863
|
|
|
|
|
|
|
elsif ( $missing eq $char[$i] or $gap eq $char[$i] ) { |
864
|
0
|
|
|
|
|
0
|
my ( $c, $s ); |
865
|
0
|
0
|
0
|
|
|
0
|
if ( $char_ids and $char_ids->[$i] ) { |
866
|
0
|
|
|
|
|
0
|
$c = $char_ids->[$i]; |
867
|
|
|
|
|
|
|
} |
868
|
|
|
|
|
|
|
else { |
869
|
0
|
|
|
|
|
0
|
$c = $i; |
870
|
|
|
|
|
|
|
} |
871
|
0
|
0
|
0
|
|
|
0
|
if ( $special and $special->{ $char[$i] } ) { |
872
|
0
|
|
|
|
|
0
|
$s = $special->{ $char[$i] }; |
873
|
|
|
|
|
|
|
} |
874
|
|
|
|
|
|
|
else { |
875
|
0
|
|
|
|
|
0
|
$s = $char[$i]; |
876
|
|
|
|
|
|
|
} |
877
|
0
|
|
|
|
|
0
|
my $cell_elt = $dom->create_element( '-tag' => 'cell' ); |
878
|
0
|
|
|
|
|
0
|
$cell_elt->set_attributes( 'char' => $c ); |
879
|
0
|
|
|
|
|
0
|
$cell_elt->set_attributes( 'state' => $s ); |
880
|
0
|
|
|
|
|
0
|
$elt->set_child($cell_elt); |
881
|
|
|
|
|
|
|
} |
882
|
|
|
|
|
|
|
} |
883
|
|
|
|
|
|
|
} |
884
|
|
|
|
|
|
|
else { |
885
|
0
|
|
|
|
|
0
|
my @tmp = map { uc $_ } @char; |
|
0
|
|
|
|
|
0
|
|
886
|
0
|
|
|
|
|
0
|
my $seq = $self->get_type_object->join( \@tmp ); |
887
|
0
|
|
|
|
|
0
|
my $seq_elt = $dom->create_element( '-tag' => 'seq' ); |
888
|
|
|
|
|
|
|
#### create a text node here.... |
889
|
0
|
|
|
|
|
0
|
$seq_elt->set_text($seq); |
890
|
|
|
|
|
|
|
|
891
|
|
|
|
|
|
|
#$seq_elt->set_child( XML::LibXML::Text->new($seq) ); |
892
|
|
|
|
|
|
|
#### |
893
|
0
|
|
|
|
|
0
|
$elt->set_child($seq_elt); |
894
|
|
|
|
|
|
|
} |
895
|
0
|
|
|
|
|
0
|
return $elt; |
896
|
|
|
|
|
|
|
} |
897
|
|
|
|
|
|
|
|
898
|
|
|
|
|
|
|
=item copy_atts() |
899
|
|
|
|
|
|
|
|
900
|
|
|
|
|
|
|
Not implemented! |
901
|
|
|
|
|
|
|
|
902
|
|
|
|
|
|
|
=cut |
903
|
|
|
|
|
|
|
|
904
|
|
|
|
0
|
1
|
|
sub copy_atts { } # XXX not implemented |
905
|
|
|
|
|
|
|
|
906
|
|
|
|
|
|
|
=item complement() |
907
|
|
|
|
|
|
|
|
908
|
|
|
|
|
|
|
Not implemented! |
909
|
|
|
|
|
|
|
|
910
|
|
|
|
|
|
|
=cut |
911
|
|
|
|
|
|
|
|
912
|
|
|
|
0
|
1
|
|
sub complement { } # XXX not implemented |
913
|
|
|
|
|
|
|
|
914
|
|
|
|
|
|
|
=item slice() |
915
|
|
|
|
|
|
|
|
916
|
|
|
|
|
|
|
Not implemented! |
917
|
|
|
|
|
|
|
|
918
|
|
|
|
|
|
|
=cut |
919
|
|
|
|
|
|
|
|
920
|
|
|
|
|
|
|
sub slice { # XXX not implemented |
921
|
0
|
|
|
0
|
1
|
0
|
my $self = shift; |
922
|
0
|
|
|
|
|
0
|
my $start = int $_[0]; |
923
|
0
|
|
|
|
|
0
|
my $end = int $_[1]; |
924
|
0
|
|
|
|
|
0
|
my @chars = $self->get_char; |
925
|
0
|
|
|
|
|
0
|
my $pos = $self->get_position; |
926
|
0
|
|
|
|
|
0
|
my $slice - $self->copy_atts; |
927
|
|
|
|
|
|
|
} |
928
|
1488
|
|
|
1488
|
|
4592
|
sub _type { $TYPE_CONSTANT } |
929
|
358
|
|
|
358
|
|
583
|
sub _container { $CONTAINER_CONSTANT } |
930
|
18
|
|
|
18
|
|
51
|
sub _tag { 'row' } |
931
|
|
|
|
|
|
|
|
932
|
|
|
|
|
|
|
sub _update_characters { |
933
|
133
|
|
|
133
|
|
198
|
my $self = shift; |
934
|
133
|
50
|
|
|
|
272
|
if ( my $matrix = $self->get_matrix ) { |
935
|
0
|
|
|
|
|
|
$matrix->_update_characters; |
936
|
|
|
|
|
|
|
} |
937
|
|
|
|
|
|
|
} |
938
|
|
|
|
|
|
|
} |
939
|
|
|
|
|
|
|
|
940
|
|
|
|
|
|
|
=back |
941
|
|
|
|
|
|
|
|
942
|
|
|
|
|
|
|
=cut |
943
|
|
|
|
|
|
|
|
944
|
|
|
|
|
|
|
# podinherit_insert_token |
945
|
|
|
|
|
|
|
|
946
|
|
|
|
|
|
|
=head1 SEE ALSO |
947
|
|
|
|
|
|
|
|
948
|
|
|
|
|
|
|
There is a mailing list at L<https://groups.google.com/forum/#!forum/bio-phylo> |
949
|
|
|
|
|
|
|
for any user or developer questions and discussions. |
950
|
|
|
|
|
|
|
|
951
|
|
|
|
|
|
|
=over |
952
|
|
|
|
|
|
|
|
953
|
|
|
|
|
|
|
=item L<Bio::Phylo::Taxa::TaxonLinker> |
954
|
|
|
|
|
|
|
|
955
|
|
|
|
|
|
|
This object inherits from L<Bio::Phylo::Taxa::TaxonLinker>, so the methods |
956
|
|
|
|
|
|
|
defined therein are also applicable to L<Bio::Phylo::Matrices::Datum> objects. |
957
|
|
|
|
|
|
|
|
958
|
|
|
|
|
|
|
=item L<Bio::Phylo::Matrices::TypeSafeData> |
959
|
|
|
|
|
|
|
|
960
|
|
|
|
|
|
|
This object inherits from L<Bio::Phylo::Matrices::TypeSafeData>, so the methods |
961
|
|
|
|
|
|
|
defined therein are also applicable to L<Bio::Phylo::Matrices::Datum> objects. |
962
|
|
|
|
|
|
|
|
963
|
|
|
|
|
|
|
=item L<Bio::Phylo::Manual> |
964
|
|
|
|
|
|
|
|
965
|
|
|
|
|
|
|
Also see the manual: L<Bio::Phylo::Manual> and L<http://rutgervos.blogspot.com>. |
966
|
|
|
|
|
|
|
|
967
|
|
|
|
|
|
|
=back |
968
|
|
|
|
|
|
|
|
969
|
|
|
|
|
|
|
=head1 CITATION |
970
|
|
|
|
|
|
|
|
971
|
|
|
|
|
|
|
If you use Bio::Phylo in published research, please cite it: |
972
|
|
|
|
|
|
|
|
973
|
|
|
|
|
|
|
B<Rutger A Vos>, B<Jason Caravas>, B<Klaas Hartmann>, B<Mark A Jensen> |
974
|
|
|
|
|
|
|
and B<Chase Miller>, 2011. Bio::Phylo - phyloinformatic analysis using Perl. |
975
|
|
|
|
|
|
|
I<BMC Bioinformatics> B<12>:63. |
976
|
|
|
|
|
|
|
L<http://dx.doi.org/10.1186/1471-2105-12-63> |
977
|
|
|
|
|
|
|
|
978
|
|
|
|
|
|
|
=cut |
979
|
|
|
|
|
|
|
|
980
|
|
|
|
|
|
|
1; |
981
|
|
|
|
|
|
|
__DATA__ |
982
|
|
|
|
|
|
|
|
983
|
|
|
|
|
|
|
my $DEFAULT_NAME = 'DEFAULT'; |
984
|
|
|
|
|
|
|
|
985
|
|
|
|
|
|
|
sub meta_names { |
986
|
|
|
|
|
|
|
my ($self) = @_; |
987
|
|
|
|
|
|
|
my @r; |
988
|
|
|
|
|
|
|
my $names = $self->get_generic('meta') || {}; |
989
|
|
|
|
|
|
|
foreach ( sort keys %{ $names } ) { |
990
|
|
|
|
|
|
|
push (@r, $_) unless $_ eq $DEFAULT_NAME; |
991
|
|
|
|
|
|
|
} |
992
|
|
|
|
|
|
|
unshift @r, $DEFAULT_NAME if $names->{$DEFAULT_NAME}; |
993
|
|
|
|
|
|
|
return @r; |
994
|
|
|
|
|
|
|
} |
995
|
|
|
|
|
|
|
|
996
|
|
|
|
|
|
|
sub get_SeqFeatures { $logger->warn } |
997
|
|
|
|
|
|
|
|
998
|
|
|
|
|
|
|
sub get_all_SeqFeatures { $logger->warn } |
999
|
|
|
|
|
|
|
|
1000
|
|
|
|
|
|
|
sub feature_count { $logger->warn } |
1001
|
|
|
|
|
|
|
|
1002
|
|
|
|
|
|
|
sub seq { |
1003
|
|
|
|
|
|
|
my $self = shift; |
1004
|
|
|
|
|
|
|
my $seq = $self->get_char; |
1005
|
|
|
|
|
|
|
return $seq; |
1006
|
|
|
|
|
|
|
} |
1007
|
|
|
|
|
|
|
|
1008
|
|
|
|
|
|
|
# from primary seq |
1009
|
|
|
|
|
|
|
sub subseq { |
1010
|
|
|
|
|
|
|
my ($self,$start,$end,$replace) = @_; |
1011
|
|
|
|
|
|
|
|
1012
|
|
|
|
|
|
|
if( ref($start) && $start->isa('Bio::LocationI') ) { |
1013
|
|
|
|
|
|
|
my $loc = $start; |
1014
|
|
|
|
|
|
|
$replace = $end; # do we really use this anywhere? scary. HL |
1015
|
|
|
|
|
|
|
my $seq = ""; |
1016
|
|
|
|
|
|
|
foreach my $subloc ($loc->each_Location()) { |
1017
|
|
|
|
|
|
|
my $piece = $self->subseq($subloc->start(), |
1018
|
|
|
|
|
|
|
$subloc->end(), $replace); |
1019
|
|
|
|
|
|
|
if($subloc->strand() < 0) { |
1020
|
|
|
|
|
|
|
$piece = Bio::PrimarySeq->new('-seq' => $piece)->revcom()->seq(); |
1021
|
|
|
|
|
|
|
} |
1022
|
|
|
|
|
|
|
$seq .= $piece; |
1023
|
|
|
|
|
|
|
} |
1024
|
|
|
|
|
|
|
return $seq; |
1025
|
|
|
|
|
|
|
} elsif( defined $start && defined $end ) { |
1026
|
|
|
|
|
|
|
if( $start > $end ){ |
1027
|
|
|
|
|
|
|
$self->throw("Bad start,end parameters. Start [$start] has to be ". |
1028
|
|
|
|
|
|
|
"less than end [$end]"); |
1029
|
|
|
|
|
|
|
} |
1030
|
|
|
|
|
|
|
if( $start <= 0 ) { |
1031
|
|
|
|
|
|
|
$self->throw("Bad start parameter ($start). Start must be positive."); |
1032
|
|
|
|
|
|
|
} |
1033
|
|
|
|
|
|
|
if( $end > $self->length ) { |
1034
|
|
|
|
|
|
|
$self->throw("Bad end parameter ($end). End must be less than the total length of sequence (total=".$self->length.")"); |
1035
|
|
|
|
|
|
|
} |
1036
|
|
|
|
|
|
|
|
1037
|
|
|
|
|
|
|
# remove one from start, and then length is end-start |
1038
|
|
|
|
|
|
|
$start--; |
1039
|
|
|
|
|
|
|
if( defined $replace ) { |
1040
|
|
|
|
|
|
|
return substr( $self->seq(), $start, ($end-$start), $replace); |
1041
|
|
|
|
|
|
|
} else { |
1042
|
|
|
|
|
|
|
return substr( $self->seq(), $start, ($end-$start)); |
1043
|
|
|
|
|
|
|
} |
1044
|
|
|
|
|
|
|
} else { |
1045
|
|
|
|
|
|
|
$self->warn("Incorrect parameters to subseq - must be two integers or a Bio::LocationI object. Got:", $self,$start,$end,$replace); |
1046
|
|
|
|
|
|
|
return; |
1047
|
|
|
|
|
|
|
} |
1048
|
|
|
|
|
|
|
} |
1049
|
|
|
|
|
|
|
|
1050
|
|
|
|
|
|
|
sub write_GFF { $logger->warn } |
1051
|
|
|
|
|
|
|
|
1052
|
|
|
|
|
|
|
sub annotation { $logger->warn } |
1053
|
|
|
|
|
|
|
|
1054
|
|
|
|
|
|
|
sub species { $logger->warn } |
1055
|
|
|
|
|
|
|
|
1056
|
|
|
|
|
|
|
sub primary_seq { $logger->warn } |
1057
|
|
|
|
|
|
|
|
1058
|
|
|
|
|
|
|
sub accession_number { $logger->warn } |
1059
|
|
|
|
|
|
|
|
1060
|
|
|
|
|
|
|
sub alphabet { |
1061
|
|
|
|
|
|
|
my $self = shift; |
1062
|
|
|
|
|
|
|
my $type = $self->get_type; |
1063
|
|
|
|
|
|
|
return lc $type; |
1064
|
|
|
|
|
|
|
} |
1065
|
|
|
|
|
|
|
|
1066
|
|
|
|
|
|
|
sub can_call_new { $logger->warn } |
1067
|
|
|
|
|
|
|
|
1068
|
|
|
|
|
|
|
sub desc { |
1069
|
|
|
|
|
|
|
my ( $self, $desc ) = @_; |
1070
|
|
|
|
|
|
|
if ( defined $desc ) { |
1071
|
|
|
|
|
|
|
$self->set_desc( $desc ); |
1072
|
|
|
|
|
|
|
} |
1073
|
|
|
|
|
|
|
return $self->get_desc; |
1074
|
|
|
|
|
|
|
} |
1075
|
|
|
|
|
|
|
|
1076
|
|
|
|
|
|
|
sub display_id { shift->get_name } |
1077
|
|
|
|
|
|
|
|
1078
|
|
|
|
|
|
|
sub id { shift->get_name } |
1079
|
|
|
|
|
|
|
|
1080
|
|
|
|
|
|
|
sub is_circular { $logger->warn } |
1081
|
|
|
|
|
|
|
|
1082
|
|
|
|
|
|
|
sub length { shift->get_length } |
1083
|
|
|
|
|
|
|
|
1084
|
|
|
|
|
|
|
sub moltype { shift->alphabet } |
1085
|
|
|
|
|
|
|
|
1086
|
|
|
|
|
|
|
sub primary_id { $logger->warn } |
1087
|
|
|
|
|
|
|
|
1088
|
|
|
|
|
|
|
sub revcom { $logger->warn } |
1089
|
|
|
|
|
|
|
|
1090
|
|
|
|
|
|
|
sub translate { $logger->warn } |
1091
|
|
|
|
|
|
|
|
1092
|
|
|
|
|
|
|
sub trunc { $logger->warn } |
1093
|
|
|
|
|
|
|
|
1094
|
|
|
|
|
|
|
sub get_nse{ |
1095
|
|
|
|
|
|
|
my ($self,$char1,$char2) = @_; |
1096
|
|
|
|
|
|
|
|
1097
|
|
|
|
|
|
|
$char1 ||= "/"; |
1098
|
|
|
|
|
|
|
$char2 ||= "-"; |
1099
|
|
|
|
|
|
|
|
1100
|
|
|
|
|
|
|
$self->throw("Attribute id not set") unless defined($self->id()); |
1101
|
|
|
|
|
|
|
$self->throw("Attribute start not set") unless defined($self->start()); |
1102
|
|
|
|
|
|
|
$self->throw("Attribute end not set") unless defined($self->end()); |
1103
|
|
|
|
|
|
|
|
1104
|
|
|
|
|
|
|
return $self->id() . $char1 . $self->start . $char2 . $self->end ; |
1105
|
|
|
|
|
|
|
|
1106
|
|
|
|
|
|
|
} |
1107
|
|
|
|
|
|
|
|
1108
|
|
|
|
|
|
|
sub strand { |
1109
|
|
|
|
|
|
|
my ( $self, $strand ) = @_; |
1110
|
|
|
|
|
|
|
if ( defined $strand ) { |
1111
|
|
|
|
|
|
|
$self->set_generic( 'strand' => $strand ); |
1112
|
|
|
|
|
|
|
} |
1113
|
|
|
|
|
|
|
return $self->get_generic( 'strand' ); |
1114
|
|
|
|
|
|
|
} |
1115
|
|
|
|
|
|
|
|
1116
|
|
|
|
|
|
|
sub start { |
1117
|
|
|
|
|
|
|
my ( $self, $start ) = @_; |
1118
|
|
|
|
|
|
|
if ( defined $start ) { |
1119
|
|
|
|
|
|
|
$self->set_position( $start ); |
1120
|
|
|
|
|
|
|
} |
1121
|
|
|
|
|
|
|
return $self->get_position; |
1122
|
|
|
|
|
|
|
} |
1123
|
|
|
|
|
|
|
|
1124
|
|
|
|
|
|
|
sub end { |
1125
|
|
|
|
|
|
|
my ( $self, $end ) = @_; |
1126
|
|
|
|
|
|
|
if ( defined $end ) { |
1127
|
|
|
|
|
|
|
$self->set_generic( 'end' => $end ); |
1128
|
|
|
|
|
|
|
} |
1129
|
|
|
|
|
|
|
$end = $self->get_generic( 'end' ); |
1130
|
|
|
|
|
|
|
if ( defined $end ) { |
1131
|
|
|
|
|
|
|
return $end; |
1132
|
|
|
|
|
|
|
} |
1133
|
|
|
|
|
|
|
else { |
1134
|
|
|
|
|
|
|
return scalar( @{ $self->get_entities } ) + $self->get_position - 1; |
1135
|
|
|
|
|
|
|
} |
1136
|
|
|
|
|
|
|
} |