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| 1 |  |  |  |  |  |  | package Bio::MUST::Drivers::Mafft; | 
| 2 |  |  |  |  |  |  | # ABSTRACT: Bio::MUST driver for running the MAFFT program | 
| 3 |  |  |  |  |  |  | # CONTRIBUTOR: Amandine BERTRAND <amandine.bertrand@doct.uliege.be> | 
| 4 |  |  |  |  |  |  | $Bio::MUST::Drivers::Mafft::VERSION = '0.191910'; | 
| 5 | 6 |  |  | 6 |  | 4864345 | use Moose; | 
|  | 6 |  |  |  |  | 23 |  | 
|  | 6 |  |  |  |  | 60 |  | 
| 6 | 6 |  |  | 6 |  | 43599 | use namespace::autoclean; | 
|  | 6 |  |  |  |  | 18 |  | 
|  | 6 |  |  |  |  | 57 |  | 
| 7 |  |  |  |  |  |  |  | 
| 8 | 6 |  |  | 6 |  | 625 | use autodie; | 
|  | 6 |  |  |  |  | 18 |  | 
|  | 6 |  |  |  |  | 58 |  | 
| 9 | 6 |  |  | 6 |  | 34104 | use feature qw(say); | 
|  | 6 |  |  |  |  | 25 |  | 
|  | 6 |  |  |  |  | 577 |  | 
| 10 |  |  |  |  |  |  |  | 
| 11 |  |  |  |  |  |  | # use Smart::Comments; | 
| 12 |  |  |  |  |  |  |  | 
| 13 | 6 |  |  | 6 |  | 47 | use Carp; | 
|  | 6 |  |  |  |  | 20 |  | 
|  | 6 |  |  |  |  | 548 |  | 
| 14 | 6 |  |  | 6 |  | 50 | use IPC::System::Simple qw(system); | 
|  | 6 |  |  |  |  | 13 |  | 
|  | 6 |  |  |  |  | 331 |  | 
| 15 | 6 |  |  | 6 |  | 40 | use Module::Runtime qw(use_module); | 
|  | 6 |  |  |  |  | 24 |  | 
|  | 6 |  |  |  |  | 49 |  | 
| 16 | 6 |  |  | 6 |  | 473 | use Path::Class qw(file); | 
|  | 6 |  |  |  |  | 12 |  | 
|  | 6 |  |  |  |  | 314 |  | 
| 17 |  |  |  |  |  |  |  | 
| 18 | 6 |  |  | 6 |  | 59 | use Bio::MUST::Core; | 
|  | 6 |  |  |  |  | 20 |  | 
|  | 6 |  |  |  |  | 316 |  | 
| 19 |  |  |  |  |  |  | extends 'Bio::FastParsers::Base'; | 
| 20 |  |  |  |  |  |  |  | 
| 21 | 6 |  |  | 6 |  | 560 | use Bio::MUST::Drivers::Utils qw(stringify_args); | 
|  | 6 |  |  |  |  | 18 |  | 
|  | 6 |  |  |  |  | 371 |  | 
| 22 | 6 |  |  | 6 |  | 43 | use aliased 'Bio::MUST::Core::Ali'; | 
|  | 6 |  |  |  |  | 11 |  | 
|  | 6 |  |  |  |  | 50 |  | 
| 23 |  |  |  |  |  |  |  | 
| 24 |  |  |  |  |  |  |  | 
| 25 |  |  |  |  |  |  | sub align_all {                             ## no critic (RequireArgUnpacking) | 
| 26 | 0 |  |  | 0 | 0 |  | return shift->_mafft('align_all', @_); | 
| 27 |  |  |  |  |  |  | } | 
| 28 |  |  |  |  |  |  |  | 
| 29 |  |  |  |  |  |  | sub seqs2profile {                          ## no critic (RequireArgUnpacking) | 
| 30 | 0 |  |  | 0 | 0 |  | return shift->_mafft('seqs2profile', @_); | 
| 31 |  |  |  |  |  |  | } | 
| 32 |  |  |  |  |  |  |  | 
| 33 |  |  |  |  |  |  | sub profile2profile {                       ## no critic (RequireArgUnpacking) | 
| 34 | 0 |  |  | 0 | 0 |  | my $out = shift->_mafft('profile2profile' , @_); | 
| 35 | 0 | 0 |  |  |  |  | return $out if $out; | 
| 36 |  |  |  |  |  |  |  | 
| 37 | 0 |  |  |  |  |  | carp '[BMD] Warning: cannot align profiles; returning nothing!'; | 
| 38 | 0 |  |  |  |  |  | return; | 
| 39 |  |  |  |  |  |  | } | 
| 40 |  |  |  |  |  |  |  | 
| 41 |  |  |  |  |  |  | sub _mafft { | 
| 42 | 0 |  |  | 0 |  |  | my $self    = shift; | 
| 43 | 0 |  |  |  |  |  | my $mode    = shift; | 
| 44 | 0 |  |  |  |  |  | my $profile;            # conditional declaring is bad... | 
| 45 | 0 | 0 |  |  |  |  | $profile = shift unless $mode eq 'align_all'; | 
| 46 | 0 |  | 0 |  |  |  | my $args    = shift // {}; | 
| 47 |  |  |  |  |  |  |  | 
| 48 |  |  |  |  |  |  | #### in _mafft | 
| 49 |  |  |  |  |  |  |  | 
| 50 |  |  |  |  |  |  | # provision executable | 
| 51 | 0 |  |  |  |  |  | my $app = use_module('Bio::MUST::Provision::Mafft')->new; | 
| 52 | 0 |  |  |  |  |  | $app->meet(); | 
| 53 |  |  |  |  |  |  |  | 
| 54 |  |  |  |  |  |  | # setup input/output files | 
| 55 | 0 |  |  |  |  |  | my $infile  = $self->filename; | 
| 56 | 0 |  |  |  |  |  | my $outfile = $infile . '.mafft'; | 
| 57 |  |  |  |  |  |  |  | 
| 58 | 0 | 0 |  |  |  |  | $args->{$profile} = undef if $profile;      # should come last (no --) | 
| 59 | 0 |  |  |  |  |  | my $args_str  = stringify_args($args); | 
| 60 |  |  |  |  |  |  |  | 
| 61 | 0 |  |  |  |  |  | my %opt_for = ( | 
| 62 |  |  |  |  |  |  | align_all       => q{}, | 
| 63 |  |  |  |  |  |  | seqs2profile    => '--add', | 
| 64 |  |  |  |  |  |  | profile2profile => '--addprofile', | 
| 65 |  |  |  |  |  |  | ); | 
| 66 |  |  |  |  |  |  |  | 
| 67 |  |  |  |  |  |  | # create mafft command | 
| 68 | 0 |  |  |  |  |  | my $pgm = 'mafft';      # linsi, ginsi,... do not work | 
| 69 | 0 |  |  |  |  |  | my $cmd = "$pgm $opt_for{$mode} $infile $args_str > $outfile 2> /dev/null"; | 
| 70 |  |  |  |  |  |  | #### $cmd | 
| 71 |  |  |  |  |  |  |  | 
| 72 |  |  |  |  |  |  | # try to robustly execute mafft | 
| 73 | 0 |  |  |  |  |  | my $ret_code = system( [ 0, 1, 127 ], $cmd); | 
| 74 | 0 | 0 |  |  |  |  | if ($ret_code == 127) { | 
| 75 | 0 |  |  |  |  |  | carp "[BMD] Warning: cannot execute $pgm command; returning nothing!"; | 
| 76 | 0 |  |  |  |  |  | return; | 
| 77 |  |  |  |  |  |  | } | 
| 78 | 0 | 0 |  |  |  |  | if ($ret_code == 1) { | 
| 79 | 0 |  |  |  |  |  | carp "[BMD] Warning: $pgm cannot align files; returning nothing!"; | 
| 80 | 0 |  |  |  |  |  | file($outfile)->remove;                 # ugly but needed | 
| 81 | 0 |  |  |  |  |  | return; | 
| 82 |  |  |  |  |  |  | } | 
| 83 |  |  |  |  |  |  | # TODO: try to bypass shell (need for absolute path to executable then) | 
| 84 |  |  |  |  |  |  |  | 
| 85 | 0 |  |  |  |  |  | my $out = Ali->load($outfile); | 
| 86 |  |  |  |  |  |  |  | 
| 87 |  |  |  |  |  |  | # unlink temp files | 
| 88 | 0 |  |  |  |  |  | file($outfile)->remove; | 
| 89 |  |  |  |  |  |  |  | 
| 90 |  |  |  |  |  |  | # return Ali | 
| 91 | 0 |  |  |  |  |  | return $out; | 
| 92 |  |  |  |  |  |  | } | 
| 93 |  |  |  |  |  |  |  | 
| 94 |  |  |  |  |  |  |  | 
| 95 |  |  |  |  |  |  |  | 
| 96 |  |  |  |  |  |  | __PACKAGE__->meta->make_immutable; | 
| 97 |  |  |  |  |  |  | 1; | 
| 98 |  |  |  |  |  |  |  | 
| 99 |  |  |  |  |  |  | __END__ | 
| 100 |  |  |  |  |  |  |  | 
| 101 |  |  |  |  |  |  | =pod | 
| 102 |  |  |  |  |  |  |  | 
| 103 |  |  |  |  |  |  | =head1 NAME | 
| 104 |  |  |  |  |  |  |  | 
| 105 |  |  |  |  |  |  | Bio::MUST::Drivers::Mafft - Bio::MUST driver for running the MAFFT program | 
| 106 |  |  |  |  |  |  |  | 
| 107 |  |  |  |  |  |  | =head1 VERSION | 
| 108 |  |  |  |  |  |  |  | 
| 109 |  |  |  |  |  |  | version 0.191910 | 
| 110 |  |  |  |  |  |  |  | 
| 111 |  |  |  |  |  |  | =head1 SYNOPSIS | 
| 112 |  |  |  |  |  |  |  | 
| 113 |  |  |  |  |  |  | # TODO | 
| 114 |  |  |  |  |  |  |  | 
| 115 |  |  |  |  |  |  | =head1 DESCRIPTION | 
| 116 |  |  |  |  |  |  |  | 
| 117 |  |  |  |  |  |  | # TODO | 
| 118 |  |  |  |  |  |  |  | 
| 119 |  |  |  |  |  |  | =head1 AUTHOR | 
| 120 |  |  |  |  |  |  |  | 
| 121 |  |  |  |  |  |  | Denis BAURAIN <denis.baurain@uliege.be> | 
| 122 |  |  |  |  |  |  |  | 
| 123 |  |  |  |  |  |  | =head1 CONTRIBUTOR | 
| 124 |  |  |  |  |  |  |  | 
| 125 |  |  |  |  |  |  | =for stopwords Amandine BERTRAND | 
| 126 |  |  |  |  |  |  |  | 
| 127 |  |  |  |  |  |  | Amandine BERTRAND <amandine.bertrand@doct.uliege.be> | 
| 128 |  |  |  |  |  |  |  | 
| 129 |  |  |  |  |  |  | =head1 COPYRIGHT AND LICENSE | 
| 130 |  |  |  |  |  |  |  | 
| 131 |  |  |  |  |  |  | This software is copyright (c) 2013 by University of Liege / Unit of Eukaryotic Phylogenomics / Denis BAURAIN. | 
| 132 |  |  |  |  |  |  |  | 
| 133 |  |  |  |  |  |  | This is free software; you can redistribute it and/or modify it under | 
| 134 |  |  |  |  |  |  | the same terms as the Perl 5 programming language system itself. | 
| 135 |  |  |  |  |  |  |  | 
| 136 |  |  |  |  |  |  | =cut |