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| 1 |  |  |  |  |  |  | package Bio::MUST::Apps::TwoScalp; | 
| 2 |  |  |  |  |  |  | # ABSTRACT: Main class for two-scalp tool | 
| 3 |  |  |  |  |  |  | $Bio::MUST::Apps::TwoScalp::VERSION = '0.231010'; | 
| 4 | 1 |  |  | 1 |  | 263349 | use strict; | 
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| 5 | 1 |  |  | 1 |  | 6 | use warnings; | 
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| 6 |  |  |  |  |  |  |  | 
| 7 | 1 |  |  | 1 |  | 577 | use Bio::MUST::Apps::SlaveAligner::Local; | 
|  | 1 |  |  |  |  | 4767058 |  | 
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| 8 | 1 |  |  | 1 |  | 724 | use Bio::MUST::Apps::TwoScalp::Seq2Seq; | 
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| 9 | 1 |  |  | 1 |  | 691 | use Bio::MUST::Apps::TwoScalp::Profile2Profile; | 
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| 10 | 1 |  |  | 1 |  | 686 | use Bio::MUST::Apps::TwoScalp::Seqs2Profile; | 
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| 11 | 1 |  |  | 1 |  | 571 | use Bio::MUST::Apps::TwoScalp::AlignAll; | 
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| 13 |  |  |  |  |  |  | 1; | 
| 14 |  |  |  |  |  |  |  | 
| 15 |  |  |  |  |  |  | __END__ | 
| 16 |  |  |  |  |  |  |  | 
| 17 |  |  |  |  |  |  | =pod | 
| 18 |  |  |  |  |  |  |  | 
| 19 |  |  |  |  |  |  | =head1 NAME | 
| 20 |  |  |  |  |  |  |  | 
| 21 |  |  |  |  |  |  | Bio::MUST::Apps::TwoScalp - Main class for two-scalp tool | 
| 22 |  |  |  |  |  |  |  | 
| 23 |  |  |  |  |  |  | =head1 VERSION | 
| 24 |  |  |  |  |  |  |  | 
| 25 |  |  |  |  |  |  | version 0.231010 | 
| 26 |  |  |  |  |  |  |  | 
| 27 |  |  |  |  |  |  | =head1 SYNOPSIS | 
| 28 |  |  |  |  |  |  |  | 
| 29 |  |  |  |  |  |  | # get documentation | 
| 30 |  |  |  |  |  |  | $ two-scalp.pl --man | 
| 31 |  |  |  |  |  |  |  | 
| 32 |  |  |  |  |  |  | # align unaligned sequences within provided example ALI file | 
| 33 |  |  |  |  |  |  | $ two-scalp.pl test/PTHR22663.ali --out=-ts | 
| 34 |  |  |  |  |  |  |  | 
| 35 |  |  |  |  |  |  | =head1 DESCRIPTION | 
| 36 |  |  |  |  |  |  |  | 
| 37 |  |  |  |  |  |  | C<two-scapl.pl> is an application to align or re-align sequences in existing | 
| 38 |  |  |  |  |  |  | multiple sequences alignments (FASTA or ALI file formats). Its main engine is | 
| 39 |  |  |  |  |  |  | BLAST L<https://blast.ncbi.nlm.nih.gov/>. | 
| 40 |  |  |  |  |  |  |  | 
| 41 |  |  |  |  |  |  | Note that only alignable regions of the sequences are added to the alignment, | 
| 42 |  |  |  |  |  |  | which may lead to discarding low-conserved regions. Moreover, some sequences | 
| 43 |  |  |  |  |  |  | can generate multiple aligned fragments (BLAST HSPs). If you do not like this | 
| 44 |  |  |  |  |  |  | behavior, C<two-scalp.pl> is not for you! | 
| 45 |  |  |  |  |  |  |  | 
| 46 |  |  |  |  |  |  | =head1 AUTHOR | 
| 47 |  |  |  |  |  |  |  | 
| 48 |  |  |  |  |  |  | Denis BAURAIN <denis.baurain@uliege.be> | 
| 49 |  |  |  |  |  |  |  | 
| 50 |  |  |  |  |  |  | =head1 COPYRIGHT AND LICENSE | 
| 51 |  |  |  |  |  |  |  | 
| 52 |  |  |  |  |  |  | This software is copyright (c) 2013 by University of Liege / Unit of Eukaryotic Phylogenomics / Denis BAURAIN. | 
| 53 |  |  |  |  |  |  |  | 
| 54 |  |  |  |  |  |  | This is free software; you can redistribute it and/or modify it under | 
| 55 |  |  |  |  |  |  | the same terms as the Perl 5 programming language system itself. | 
| 56 |  |  |  |  |  |  |  | 
| 57 |  |  |  |  |  |  | =cut |