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| 1 |  |  |  |  |  |  | # ABSTRACT: Front-end class for tabular HMMER parser | 
| 2 |  |  |  |  |  |  | # CONTRIBUTOR: Arnaud DI FRANCO <arnaud.difranco@gmail.com> | 
| 3 |  |  |  |  |  |  | $Bio::FastParsers::Hmmer::Table::VERSION = '0.221230'; | 
| 4 |  |  |  |  |  |  | use Moose; | 
| 5 | 7 |  |  | 7 |  | 51 | use namespace::autoclean; | 
|  | 7 |  |  |  |  | 15 |  | 
|  | 7 |  |  |  |  | 51 |  | 
| 6 | 7 |  |  | 7 |  | 43073 |  | 
|  | 7 |  |  |  |  | 21 |  | 
|  | 7 |  |  |  |  | 66 |  | 
| 7 |  |  |  |  |  |  | use autodie; | 
| 8 | 7 |  |  | 7 |  | 647 |  | 
|  | 7 |  |  |  |  | 16 |  | 
|  | 7 |  |  |  |  | 67 |  | 
| 9 |  |  |  |  |  |  | use List::AllUtils qw(mesh); | 
| 10 | 7 |  |  | 7 |  | 33714 |  | 
|  | 7 |  |  |  |  | 15 |  | 
|  | 7 |  |  |  |  | 595 |  | 
| 11 |  |  |  |  |  |  | extends 'Bio::FastParsers::Base'; | 
| 12 |  |  |  |  |  |  |  | 
| 13 |  |  |  |  |  |  | use Bio::FastParsers::Constants qw(:files); | 
| 14 | 7 |  |  | 7 |  | 53 | use aliased 'Bio::FastParsers::Hmmer::Table::Hit'; | 
|  | 7 |  |  |  |  | 20 |  | 
|  | 7 |  |  |  |  | 927 |  | 
| 15 | 7 |  |  | 7 |  | 62 |  | 
|  | 7 |  |  |  |  | 15 |  | 
|  | 7 |  |  |  |  | 60 |  | 
| 16 |  |  |  |  |  |  |  | 
| 17 |  |  |  |  |  |  | # public attributes (inherited) | 
| 18 |  |  |  |  |  |  |  | 
| 19 |  |  |  |  |  |  |  | 
| 20 |  |  |  |  |  |  | # private attributes | 
| 21 |  |  |  |  |  |  |  | 
| 22 |  |  |  |  |  |  | has '_line_iterator' => ( | 
| 23 |  |  |  |  |  |  | traits   => ['Code'], | 
| 24 |  |  |  |  |  |  | is       => 'ro', | 
| 25 |  |  |  |  |  |  | isa      => 'CodeRef', | 
| 26 |  |  |  |  |  |  | init_arg => undef, | 
| 27 |  |  |  |  |  |  | lazy     => 1, | 
| 28 |  |  |  |  |  |  | builder  => '_build_line_iterator', | 
| 29 |  |  |  |  |  |  | handles  => { | 
| 30 |  |  |  |  |  |  | _next_line => 'execute', | 
| 31 |  |  |  |  |  |  | }, | 
| 32 |  |  |  |  |  |  | ); | 
| 33 |  |  |  |  |  |  |  | 
| 34 |  |  |  |  |  |  | ## no critic (ProhibitUnusedPrivateSubroutines) | 
| 35 |  |  |  |  |  |  |  | 
| 36 |  |  |  |  |  |  | my $self = shift; | 
| 37 |  |  |  |  |  |  |  | 
| 38 | 2 |  |  | 2 |  | 6 | open my $fh, '<', $self->file;      # autodie | 
| 39 |  |  |  |  |  |  | return sub { <$fh> };               # return closure | 
| 40 | 2 |  |  |  |  | 63 | } | 
| 41 | 2 |  |  | 66 |  | 2293 |  | 
|  | 66 |  |  |  |  | 522 |  | 
| 42 |  |  |  |  |  |  | ## use critic | 
| 43 |  |  |  |  |  |  |  | 
| 44 |  |  |  |  |  |  | my @attrs = qw( | 
| 45 |  |  |  |  |  |  | target_name target_accession | 
| 46 |  |  |  |  |  |  | query_name  query_accession | 
| 47 |  |  |  |  |  |  | evalue          score          bias | 
| 48 |  |  |  |  |  |  | best_dom_evalue best_dom_score best_dom_bias | 
| 49 |  |  |  |  |  |  | exp reg clu ov env dom rep inc | 
| 50 |  |  |  |  |  |  | target_description | 
| 51 |  |  |  |  |  |  | );      # DID try to use MOP to get Hit attrs but order was not preserved | 
| 52 |  |  |  |  |  |  |  | 
| 53 |  |  |  |  |  |  |  | 
| 54 |  |  |  |  |  |  | my $self = shift; | 
| 55 |  |  |  |  |  |  |  | 
| 56 |  |  |  |  |  |  | LINE: | 
| 57 | 60 |  |  | 60 | 1 | 119 | while (my $line = $self->_next_line) { | 
| 58 |  |  |  |  |  |  |  | 
| 59 |  |  |  |  |  |  | # skip header/comments and empty lines | 
| 60 | 60 |  |  |  |  | 2082 | chomp $line; | 
| 61 |  |  |  |  |  |  | next LINE if $line =~ $COMMENT_LINE | 
| 62 |  |  |  |  |  |  | || $line =~ $EMPTY_LINE; | 
| 63 | 64 |  |  |  |  | 158 |  | 
| 64 | 64 | 100 | 66 |  |  | 879 | # process Hit line | 
| 65 |  |  |  |  |  |  | my @fields = split(/\s+/xms, $line, 19); | 
| 66 |  |  |  |  |  |  |  | 
| 67 |  |  |  |  |  |  | # Fields | 
| 68 | 58 |  |  |  |  | 670 | #   0.  target name | 
| 69 |  |  |  |  |  |  | #   1.  accession | 
| 70 |  |  |  |  |  |  | #   2.  query name | 
| 71 |  |  |  |  |  |  | #   3.  accession | 
| 72 |  |  |  |  |  |  | #   4.  E-value | 
| 73 |  |  |  |  |  |  | #   5.  score | 
| 74 |  |  |  |  |  |  | #   6.  bias | 
| 75 |  |  |  |  |  |  | #   7.  E-value | 
| 76 |  |  |  |  |  |  | #   8.  score | 
| 77 |  |  |  |  |  |  | #   9.  bias | 
| 78 |  |  |  |  |  |  | #  10.  exp | 
| 79 |  |  |  |  |  |  | #  11.  reg | 
| 80 |  |  |  |  |  |  | #  12.  clu | 
| 81 |  |  |  |  |  |  | #  13.  ov | 
| 82 |  |  |  |  |  |  | #  14.  env | 
| 83 |  |  |  |  |  |  | #  15.  dom | 
| 84 |  |  |  |  |  |  | #  16.  rep | 
| 85 |  |  |  |  |  |  | #  17.  inc | 
| 86 |  |  |  |  |  |  | #  18.  description of target | 
| 87 |  |  |  |  |  |  |  | 
| 88 |  |  |  |  |  |  | # coerce numeric fields to numbers | 
| 89 |  |  |  |  |  |  | @fields[4..17] = map { 0 + $_ } @fields[4..17]; | 
| 90 |  |  |  |  |  |  |  | 
| 91 |  |  |  |  |  |  | # set missing field values to undef | 
| 92 | 58 |  |  |  |  | 260 | @fields[1,3,18] = map { $_ eq '-' ? undef : $_ } @fields[1,3,18]; | 
|  | 812 |  |  |  |  | 1454 |  | 
| 93 |  |  |  |  |  |  |  | 
| 94 |  |  |  |  |  |  | # return Hit object | 
| 95 | 58 | 100 |  |  |  | 155 | return Hit->new( { mesh @attrs, @fields } ); | 
|  | 174 |  |  |  |  | 392 |  | 
| 96 |  |  |  |  |  |  | } | 
| 97 |  |  |  |  |  |  |  | 
| 98 | 58 |  |  |  |  | 3031 | return;                         # no more line to read | 
| 99 |  |  |  |  |  |  | } | 
| 100 |  |  |  |  |  |  |  | 
| 101 | 2 |  |  |  |  | 11 | __PACKAGE__->meta->make_immutable; | 
| 102 |  |  |  |  |  |  | 1; | 
| 103 |  |  |  |  |  |  |  | 
| 104 |  |  |  |  |  |  |  | 
| 105 |  |  |  |  |  |  | =pod | 
| 106 |  |  |  |  |  |  |  | 
| 107 |  |  |  |  |  |  | =head1 NAME | 
| 108 |  |  |  |  |  |  |  | 
| 109 |  |  |  |  |  |  | Bio::FastParsers::Hmmer::Table - Front-end class for tabular HMMER parser | 
| 110 |  |  |  |  |  |  |  | 
| 111 |  |  |  |  |  |  | =head1 VERSION | 
| 112 |  |  |  |  |  |  |  | 
| 113 |  |  |  |  |  |  | version 0.221230 | 
| 114 |  |  |  |  |  |  |  | 
| 115 |  |  |  |  |  |  | =head1 SYNOPSIS | 
| 116 |  |  |  |  |  |  |  | 
| 117 |  |  |  |  |  |  | use aliased 'Bio::FastParsers::Hmmer::Table'; | 
| 118 |  |  |  |  |  |  |  | 
| 119 |  |  |  |  |  |  | # open and parse HMMER report in tabular format | 
| 120 |  |  |  |  |  |  | my $infile = 'test/hmmer.tblout'; | 
| 121 |  |  |  |  |  |  | my $report = Table->new( file => $infile ); | 
| 122 |  |  |  |  |  |  |  | 
| 123 |  |  |  |  |  |  | # loop through hits | 
| 124 |  |  |  |  |  |  | while (my $hit = $hit->next_hit) { | 
| 125 |  |  |  |  |  |  | my ($target_name, $evalue) = ($hit->target_name, $hit->evalue); | 
| 126 |  |  |  |  |  |  | # ... | 
| 127 |  |  |  |  |  |  | } | 
| 128 |  |  |  |  |  |  |  | 
| 129 |  |  |  |  |  |  | =head1 DESCRIPTION | 
| 130 |  |  |  |  |  |  |  | 
| 131 |  |  |  |  |  |  | # TODO | 
| 132 |  |  |  |  |  |  |  | 
| 133 |  |  |  |  |  |  | =head1 ATTRIBUTES | 
| 134 |  |  |  |  |  |  |  | 
| 135 |  |  |  |  |  |  | =head2 file | 
| 136 |  |  |  |  |  |  |  | 
| 137 |  |  |  |  |  |  | Path to HMMER report file in tabular format (--tblout) to be parsed | 
| 138 |  |  |  |  |  |  |  | 
| 139 |  |  |  |  |  |  | =head1 METHODS | 
| 140 |  |  |  |  |  |  |  | 
| 141 |  |  |  |  |  |  | =head2 next_hit | 
| 142 |  |  |  |  |  |  |  | 
| 143 |  |  |  |  |  |  | Shifts the first Hit of the report off and returns it, shortening the report | 
| 144 |  |  |  |  |  |  | by 1 and moving everything down. If there are no more Hits in the report, | 
| 145 |  |  |  |  |  |  | returns C<undef>. | 
| 146 |  |  |  |  |  |  |  | 
| 147 |  |  |  |  |  |  | # $report is a Bio::FastParsers::Hmmer::Table | 
| 148 |  |  |  |  |  |  | while (my $hit = $report->next_hit) { | 
| 149 |  |  |  |  |  |  | # process $hit | 
| 150 |  |  |  |  |  |  | # ... | 
| 151 |  |  |  |  |  |  | } | 
| 152 |  |  |  |  |  |  |  | 
| 153 |  |  |  |  |  |  | This method does not accept any arguments. | 
| 154 |  |  |  |  |  |  |  | 
| 155 |  |  |  |  |  |  | =head1 AUTHOR | 
| 156 |  |  |  |  |  |  |  | 
| 157 |  |  |  |  |  |  | Denis BAURAIN <denis.baurain@uliege.be> | 
| 158 |  |  |  |  |  |  |  | 
| 159 |  |  |  |  |  |  | =head1 CONTRIBUTOR | 
| 160 |  |  |  |  |  |  |  | 
| 161 |  |  |  |  |  |  | =for stopwords Arnaud DI FRANCO | 
| 162 |  |  |  |  |  |  |  | 
| 163 |  |  |  |  |  |  | Arnaud DI FRANCO <arnaud.difranco@gmail.com> | 
| 164 |  |  |  |  |  |  |  | 
| 165 |  |  |  |  |  |  | =head1 COPYRIGHT AND LICENSE | 
| 166 |  |  |  |  |  |  |  | 
| 167 |  |  |  |  |  |  | This software is copyright (c) 2013 by University of Liege / Unit of Eukaryotic Phylogenomics / Denis BAURAIN. | 
| 168 |  |  |  |  |  |  |  | 
| 169 |  |  |  |  |  |  | This is free software; you can redistribute it and/or modify it under | 
| 170 |  |  |  |  |  |  | the same terms as the Perl 5 programming language system itself. | 
| 171 |  |  |  |  |  |  |  | 
| 172 |  |  |  |  |  |  | =cut |