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# ABSTRACT: Front-end class for tabular HMMER parser |
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# CONTRIBUTOR: Arnaud DI FRANCO <arnaud.difranco@gmail.com> |
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$Bio::FastParsers::Hmmer::Table::VERSION = '0.221230'; |
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use Moose; |
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use namespace::autoclean; |
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use autodie; |
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use List::AllUtils qw(mesh); |
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extends 'Bio::FastParsers::Base'; |
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use Bio::FastParsers::Constants qw(:files); |
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use aliased 'Bio::FastParsers::Hmmer::Table::Hit'; |
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# public attributes (inherited) |
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# private attributes |
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has '_line_iterator' => ( |
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traits => ['Code'], |
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is => 'ro', |
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isa => 'CodeRef', |
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init_arg => undef, |
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lazy => 1, |
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builder => '_build_line_iterator', |
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handles => { |
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_next_line => 'execute', |
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}, |
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); |
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## no critic (ProhibitUnusedPrivateSubroutines) |
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my $self = shift; |
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open my $fh, '<', $self->file; # autodie |
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return sub { <$fh> }; # return closure |
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} |
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## use critic |
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my @attrs = qw( |
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target_name target_accession |
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query_name query_accession |
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evalue score bias |
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best_dom_evalue best_dom_score best_dom_bias |
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exp reg clu ov env dom rep inc |
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target_description |
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); # DID try to use MOP to get Hit attrs but order was not preserved |
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my $self = shift; |
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LINE: |
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while (my $line = $self->_next_line) { |
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# skip header/comments and empty lines |
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chomp $line; |
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next LINE if $line =~ $COMMENT_LINE |
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|| $line =~ $EMPTY_LINE; |
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# process Hit line |
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my @fields = split(/\s+/xms, $line, 19); |
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# Fields |
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# 0. target name |
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# 1. accession |
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# 2. query name |
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# 3. accession |
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# 4. E-value |
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# 5. score |
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# 6. bias |
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# 7. E-value |
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# 8. score |
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# 9. bias |
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# 10. exp |
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# 11. reg |
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# 12. clu |
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# 13. ov |
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# 14. env |
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# 15. dom |
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# 16. rep |
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# 17. inc |
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# 18. description of target |
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# coerce numeric fields to numbers |
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@fields[4..17] = map { 0 + $_ } @fields[4..17]; |
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# set missing field values to undef |
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@fields[1,3,18] = map { $_ eq '-' ? undef : $_ } @fields[1,3,18]; |
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# return Hit object |
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return Hit->new( { mesh @attrs, @fields } ); |
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} |
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return; # no more line to read |
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} |
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__PACKAGE__->meta->make_immutable; |
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1; |
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=pod |
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=head1 NAME |
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Bio::FastParsers::Hmmer::Table - Front-end class for tabular HMMER parser |
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=head1 VERSION |
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version 0.221230 |
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=head1 SYNOPSIS |
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use aliased 'Bio::FastParsers::Hmmer::Table'; |
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# open and parse HMMER report in tabular format |
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my $infile = 'test/hmmer.tblout'; |
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my $report = Table->new( file => $infile ); |
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# loop through hits |
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while (my $hit = $hit->next_hit) { |
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my ($target_name, $evalue) = ($hit->target_name, $hit->evalue); |
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# ... |
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} |
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=head1 DESCRIPTION |
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# TODO |
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=head1 ATTRIBUTES |
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=head2 file |
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Path to HMMER report file in tabular format (--tblout) to be parsed |
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=head1 METHODS |
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=head2 next_hit |
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Shifts the first Hit of the report off and returns it, shortening the report |
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by 1 and moving everything down. If there are no more Hits in the report, |
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returns C<undef>. |
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# $report is a Bio::FastParsers::Hmmer::Table |
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while (my $hit = $report->next_hit) { |
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# process $hit |
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# ... |
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} |
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This method does not accept any arguments. |
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=head1 AUTHOR |
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Denis BAURAIN <denis.baurain@uliege.be> |
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=head1 CONTRIBUTOR |
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=for stopwords Arnaud DI FRANCO |
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Arnaud DI FRANCO <arnaud.difranco@gmail.com> |
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=head1 COPYRIGHT AND LICENSE |
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This software is copyright (c) 2013 by University of Liege / Unit of Eukaryotic Phylogenomics / Denis BAURAIN. |
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This is free software; you can redistribute it and/or modify it under |
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the same terms as the Perl 5 programming language system itself. |
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=cut |