line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
1
|
|
|
|
|
|
|
# ABSTRACT: Internal class for tabular BLAST parser |
2
|
|
|
|
|
|
|
$Bio::FastParsers::Blast::Table::Hsp::VERSION = '0.221230'; |
3
|
|
|
|
|
|
|
use Moose; |
4
|
7
|
|
|
7
|
|
3535
|
use namespace::autoclean; |
|
7
|
|
|
|
|
17
|
|
|
7
|
|
|
|
|
49
|
|
5
|
7
|
|
|
7
|
|
43557
|
|
|
7
|
|
|
|
|
15
|
|
|
7
|
|
|
|
|
55
|
|
6
|
|
|
|
|
|
|
|
7
|
|
|
|
|
|
|
# public attributes |
8
|
|
|
|
|
|
|
|
9
|
|
|
|
|
|
|
has $_ => ( |
10
|
|
|
|
|
|
|
is => 'ro', |
11
|
|
|
|
|
|
|
isa => 'Str', |
12
|
|
|
|
|
|
|
required => 1, |
13
|
|
|
|
|
|
|
) for qw(query_id hit_id); |
14
|
|
|
|
|
|
|
|
15
|
|
|
|
|
|
|
has $_ => ( |
16
|
|
|
|
|
|
|
is => 'ro', |
17
|
|
|
|
|
|
|
isa => 'Num', |
18
|
|
|
|
|
|
|
required => 1, |
19
|
|
|
|
|
|
|
) for qw( |
20
|
|
|
|
|
|
|
percent_identity hsp_length mismatches gaps |
21
|
|
|
|
|
|
|
query_from query_to |
22
|
|
|
|
|
|
|
hit_from hit_to |
23
|
|
|
|
|
|
|
query_strand |
24
|
|
|
|
|
|
|
hit_strand |
25
|
|
|
|
|
|
|
query_start query_end |
26
|
|
|
|
|
|
|
hit_start hit_end |
27
|
|
|
|
|
|
|
); |
28
|
|
|
|
|
|
|
|
29
|
|
|
|
|
|
|
has $_ => ( |
30
|
|
|
|
|
|
|
is => 'ro', |
31
|
|
|
|
|
|
|
isa => 'Maybe[Num]', |
32
|
|
|
|
|
|
|
required => 1, |
33
|
|
|
|
|
|
|
) for qw( |
34
|
|
|
|
|
|
|
evalue bit_score |
35
|
|
|
|
|
|
|
); |
36
|
|
|
|
|
|
|
|
37
|
|
|
|
|
|
|
|
38
|
|
|
|
|
|
|
|
39
|
|
|
|
|
|
|
return shift->evalue |
40
|
|
|
|
|
|
|
} |
41
|
|
|
|
|
|
|
|
42
|
0
|
|
|
0
|
1
|
|
|
43
|
|
|
|
|
|
|
__PACKAGE__->meta->make_immutable; |
44
|
|
|
|
|
|
|
1; |
45
|
|
|
|
|
|
|
|
46
|
|
|
|
|
|
|
|
47
|
|
|
|
|
|
|
=pod |
48
|
|
|
|
|
|
|
|
49
|
|
|
|
|
|
|
=head1 NAME |
50
|
|
|
|
|
|
|
|
51
|
|
|
|
|
|
|
Bio::FastParsers::Blast::Table::Hsp - Internal class for tabular BLAST parser |
52
|
|
|
|
|
|
|
|
53
|
|
|
|
|
|
|
=head1 VERSION |
54
|
|
|
|
|
|
|
|
55
|
|
|
|
|
|
|
version 0.221230 |
56
|
|
|
|
|
|
|
|
57
|
|
|
|
|
|
|
=head1 SYNOPSIS |
58
|
|
|
|
|
|
|
|
59
|
|
|
|
|
|
|
# see Bio::FastParsers::Blast::Table |
60
|
|
|
|
|
|
|
|
61
|
|
|
|
|
|
|
=head1 DESCRIPTION |
62
|
|
|
|
|
|
|
|
63
|
|
|
|
|
|
|
This class implements a single line of a tabular BLAST report. Such a line |
64
|
|
|
|
|
|
|
does not correspond to a hit but to a High-Scoring-Pair (HSP). All its methods |
65
|
|
|
|
|
|
|
are accessors. Beyond the standard fields found in the BLAST tabular output |
66
|
|
|
|
|
|
|
(e.g., C<hit_id>, C<evalue>), additional methods are available for easier |
67
|
|
|
|
|
|
|
handling of reverse strand coordinates (e.g., C<query_start>, C<hit_strand>). |
68
|
|
|
|
|
|
|
|
69
|
|
|
|
|
|
|
=head1 METHODS |
70
|
|
|
|
|
|
|
|
71
|
|
|
|
|
|
|
=head2 query_id |
72
|
|
|
|
|
|
|
|
73
|
|
|
|
|
|
|
Returns the id of the query sequence. |
74
|
|
|
|
|
|
|
|
75
|
|
|
|
|
|
|
This method does not accept any arguments. |
76
|
|
|
|
|
|
|
|
77
|
|
|
|
|
|
|
=head2 hit_id |
78
|
|
|
|
|
|
|
|
79
|
|
|
|
|
|
|
Returns the id of the hit (or subject) sequence. |
80
|
|
|
|
|
|
|
|
81
|
|
|
|
|
|
|
This method does not accept any arguments. |
82
|
|
|
|
|
|
|
|
83
|
|
|
|
|
|
|
=head2 percent_identity |
84
|
|
|
|
|
|
|
|
85
|
|
|
|
|
|
|
Returns the identity (in percents) of the HSP. |
86
|
|
|
|
|
|
|
|
87
|
|
|
|
|
|
|
This method does not accept any arguments. |
88
|
|
|
|
|
|
|
|
89
|
|
|
|
|
|
|
=head2 hsp_length |
90
|
|
|
|
|
|
|
|
91
|
|
|
|
|
|
|
Returns the length (in nt or aa) of the HSP. |
92
|
|
|
|
|
|
|
|
93
|
|
|
|
|
|
|
This method does not accept any arguments. |
94
|
|
|
|
|
|
|
|
95
|
|
|
|
|
|
|
=head2 mismatches |
96
|
|
|
|
|
|
|
|
97
|
|
|
|
|
|
|
Returns the number of mismatches of the HSP. |
98
|
|
|
|
|
|
|
|
99
|
|
|
|
|
|
|
This method does not accept any arguments. |
100
|
|
|
|
|
|
|
|
101
|
|
|
|
|
|
|
=head2 gaps |
102
|
|
|
|
|
|
|
|
103
|
|
|
|
|
|
|
Returns the number of gaps (or gap openings) of the HSP. |
104
|
|
|
|
|
|
|
|
105
|
|
|
|
|
|
|
This method does not accept any arguments. |
106
|
|
|
|
|
|
|
|
107
|
|
|
|
|
|
|
=head2 query_from |
108
|
|
|
|
|
|
|
|
109
|
|
|
|
|
|
|
Returns the HSP start in query coordinates. The value of C<query_from> is |
110
|
|
|
|
|
|
|
higher than the value of C<query_to> on reverse strands. |
111
|
|
|
|
|
|
|
|
112
|
|
|
|
|
|
|
This method does not accept any arguments. |
113
|
|
|
|
|
|
|
|
114
|
|
|
|
|
|
|
=head2 query_to |
115
|
|
|
|
|
|
|
|
116
|
|
|
|
|
|
|
Returns the HSP end in query coordinates. The value of C<query_to> is lower |
117
|
|
|
|
|
|
|
than the value of C<query_from> on reverse strands. |
118
|
|
|
|
|
|
|
|
119
|
|
|
|
|
|
|
This method does not accept any arguments. |
120
|
|
|
|
|
|
|
|
121
|
|
|
|
|
|
|
=head2 hit_from |
122
|
|
|
|
|
|
|
|
123
|
|
|
|
|
|
|
Returns the HSP start in hit (or subject) coordinates. The value of |
124
|
|
|
|
|
|
|
C<hit_from> is higher than the value of C<hit_to> on reverse strands. |
125
|
|
|
|
|
|
|
|
126
|
|
|
|
|
|
|
This method does not accept any arguments. |
127
|
|
|
|
|
|
|
|
128
|
|
|
|
|
|
|
=head2 hit_to |
129
|
|
|
|
|
|
|
|
130
|
|
|
|
|
|
|
Returns the HSP end in hit (or subject) coordinates. The value of C<hit_to> is |
131
|
|
|
|
|
|
|
lower than the value of C<hit_from> on reverse strands. |
132
|
|
|
|
|
|
|
|
133
|
|
|
|
|
|
|
This method does not accept any arguments. |
134
|
|
|
|
|
|
|
|
135
|
|
|
|
|
|
|
=head2 evalue |
136
|
|
|
|
|
|
|
|
137
|
|
|
|
|
|
|
Returns the E-value (or expect) of the HSP. |
138
|
|
|
|
|
|
|
|
139
|
|
|
|
|
|
|
This method does not accept any arguments. |
140
|
|
|
|
|
|
|
|
141
|
|
|
|
|
|
|
=head2 bit_score |
142
|
|
|
|
|
|
|
|
143
|
|
|
|
|
|
|
Returns the score (in bits) of the HSP. |
144
|
|
|
|
|
|
|
|
145
|
|
|
|
|
|
|
This method does not accept any arguments. |
146
|
|
|
|
|
|
|
|
147
|
|
|
|
|
|
|
=head2 query_strand |
148
|
|
|
|
|
|
|
|
149
|
|
|
|
|
|
|
Returns the strand (+1/-1) of the query in the HSP. |
150
|
|
|
|
|
|
|
|
151
|
|
|
|
|
|
|
This method does not accept any arguments. |
152
|
|
|
|
|
|
|
|
153
|
|
|
|
|
|
|
=head2 hit_strand |
154
|
|
|
|
|
|
|
|
155
|
|
|
|
|
|
|
Returns the strand (+1/-1) of the hit in the HSP. |
156
|
|
|
|
|
|
|
|
157
|
|
|
|
|
|
|
This method does not accept any arguments. |
158
|
|
|
|
|
|
|
|
159
|
|
|
|
|
|
|
=head2 query_start |
160
|
|
|
|
|
|
|
|
161
|
|
|
|
|
|
|
Returns the HSP start in query coordinates. The value of C<query_start> is |
162
|
|
|
|
|
|
|
guaranteed to be lower than the value of C<query_end>. |
163
|
|
|
|
|
|
|
|
164
|
|
|
|
|
|
|
This method does not accept any arguments. |
165
|
|
|
|
|
|
|
|
166
|
|
|
|
|
|
|
=head2 query_end |
167
|
|
|
|
|
|
|
|
168
|
|
|
|
|
|
|
Returns the HSP end in query coordinates. The value of C<query_end> is |
169
|
|
|
|
|
|
|
guaranteed to be higher than the value of C<query_start>. |
170
|
|
|
|
|
|
|
|
171
|
|
|
|
|
|
|
This method does not accept any arguments. |
172
|
|
|
|
|
|
|
|
173
|
|
|
|
|
|
|
=head2 hit_start |
174
|
|
|
|
|
|
|
|
175
|
|
|
|
|
|
|
Returns the HSP start in hit (or subject) coordinates. The value of |
176
|
|
|
|
|
|
|
C<hit_start> is guaranteed to be lower than the value of C<hit_end>. |
177
|
|
|
|
|
|
|
|
178
|
|
|
|
|
|
|
This method does not accept any arguments. |
179
|
|
|
|
|
|
|
|
180
|
|
|
|
|
|
|
=head2 hit_end |
181
|
|
|
|
|
|
|
|
182
|
|
|
|
|
|
|
Returns the HSP end in hit (or subject) coordinates. The value of C<hit_end> |
183
|
|
|
|
|
|
|
is guaranteed to be higher than the value of C<hit_start>. |
184
|
|
|
|
|
|
|
|
185
|
|
|
|
|
|
|
This method does not accept any arguments. |
186
|
|
|
|
|
|
|
|
187
|
|
|
|
|
|
|
=head1 ALIASES |
188
|
|
|
|
|
|
|
|
189
|
|
|
|
|
|
|
=head2 expect |
190
|
|
|
|
|
|
|
|
191
|
|
|
|
|
|
|
Alias for C<evalue> method. For API consistency. |
192
|
|
|
|
|
|
|
|
193
|
|
|
|
|
|
|
=head1 AUTHOR |
194
|
|
|
|
|
|
|
|
195
|
|
|
|
|
|
|
Denis BAURAIN <denis.baurain@uliege.be> |
196
|
|
|
|
|
|
|
|
197
|
|
|
|
|
|
|
=head1 COPYRIGHT AND LICENSE |
198
|
|
|
|
|
|
|
|
199
|
|
|
|
|
|
|
This software is copyright (c) 2013 by University of Liege / Unit of Eukaryotic Phylogenomics / Denis BAURAIN. |
200
|
|
|
|
|
|
|
|
201
|
|
|
|
|
|
|
This is free software; you can redistribute it and/or modify it under |
202
|
|
|
|
|
|
|
the same terms as the Perl 5 programming language system itself. |
203
|
|
|
|
|
|
|
|
204
|
|
|
|
|
|
|
=cut |