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package Bio::DOOP::SequenceFeature; |
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use strict; |
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use warnings; |
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=head1 NAME |
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Bio::DOOP::SequenceFeature - Object for the sequence features |
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=head1 VERSION |
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Version 0.10 |
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=cut |
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our $VERSION = '0.10'; |
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=head1 SYNOPSIS |
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my $seqfeat = Bio::DOOP::SequenceFeature->new($db,"112"); |
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my $type = $seqfeat->get_type; |
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=head1 DESCRIPTION |
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This object gives access to the sequence features (conserved motif, repeat, CpG island, |
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TFBS and TSS annotation). |
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=head1 AUTHORS |
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Tibor Nagy, Godollo, Hungary and Endre Sebestyen, Martonvasar, Hungary |
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=head1 METHODS |
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=head2 new |
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Creates a new sequence feature object from the sequence feature primary id. |
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Return type: Bio::DOOP::SequenceFeature object |
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$seqfeat = Bio::DOOP::SequenceFeature->new($db,"112"); |
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=cut |
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sub new { |
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my $self = {}; |
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my $dummy = shift; |
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my $db = shift; |
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my $id = shift; |
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my $ret = $db->query("SELECT * FROM sequence_feature WHERE sequence_feature_primary_id = \"$id\";"); |
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if ($#$ret == -1) { |
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return(-1); |
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} |
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my @fields = @{$$ret[0]}; |
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$self->{PRIMARY} = $fields[0]; |
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$self->{SCORE} = $fields[1]; |
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$self->{START} = $fields[2]; |
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$self->{END} = $fields[3]; |
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$self->{SEQ} = $fields[4]; |
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$self->{TYPE} = $fields[5]; |
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$self->{MOTIFID} = $fields[6]; |
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$self->{TFBSID} = $fields[7]; |
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$self->{CPGID} = $fields[8]; |
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$self->{REPEATID} = $fields[9]; |
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$self->{SSRID} = $fields[10]; |
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$self->{TSSID} = $fields[11]; |
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$self->{SUBSET_ID} = $fields[12]; |
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$self->{SEQ_ID} = $fields[13]; |
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if ($self->{TYPE} eq "ssr"){ |
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$ret = $db->query("SELECT * FROM ssr_annotation WHERE ssr_primary_id = \"".$self->{SSRID}."\";"); |
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@fields = @{$$ret[0]}; |
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$self->{SSRUNIT} = $fields[1]; |
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} |
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elsif ($self->{TYPE} eq "rep"){ |
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$ret = $db->query("SELECT * FROM repeat_annotation WHERE repeat_primary_id = \"".$self->{REPEATID}."\";"); |
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@fields = @{$$ret[0]}; |
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$self->{R_NAME} = $fields[1]; |
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$self->{R_CLASS} = $fields[2]; |
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$self->{R_DESC} = $fields[3]; |
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$self->{R_XREF} = $fields[4]; |
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} |
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elsif ($self->{TYPE} eq "tfbs"){ |
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$ret = $db->query("SELECT * FROM tfbs_annotation WHERE tfbs_primary_id = \"".$self->{TFBSID}."\";"); |
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@fields = @{$$ret[0]}; |
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$self->{TF_NAME} = $fields[1]; |
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$self->{TF_ORIG} = $fields[2]; |
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$self->{TF_DESC} = $fields[3]; |
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$self->{TF_TYPE} = $fields[4]; |
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$self->{TF_CONS} = $fields[5]; |
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$self->{TF_MTRX} = $fields[6]; |
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$self->{TF_XREF} = $fields[7]; |
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} |
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elsif ($self->{TYPE} eq "cpg"){ |
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$ret = $db->query("SELECT * FROM cpg_annotation WHERE cpg_primary_id = \"".$self->{CPGID}."\";"); |
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@fields = @{$$ret[0]}; |
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$self->{CPG_P} = $fields[1]; |
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} |
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elsif ($self->{TYPE} eq "tss"){ |
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$ret = $db->query("SELECT * FROM tss_annotation WHERE tss_primary_id = \"".$self->{TSSID}."\";"); |
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@fields = @{$$ret[0]}; |
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$self->{T_TYPE} = $fields[1]; |
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$self->{T_ID} = $fields[2]; |
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$self->{T_DESC} = $fields[3]; |
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$self->{T_XREF} = $fields[4]; |
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} |
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elsif ($self->{TYPE} eq "con"){ |
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my $motif = Bio::DOOP::Motif->new($db,$self->{MOTIFID}); |
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$self->{MOTIF} = $motif; |
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} |
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bless $self; |
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return($self); |
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} |
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=head2 get_id |
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Returns the primary ID of the feature. This is the internal ID from the MySQL database. |
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=cut |
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sub get_id { |
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my $self = shift; |
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return($self->{PRIMARY}); |
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} |
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=head2 get_score |
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This method is not yet implemented. |
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=cut |
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sub get_score { |
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my $self = shift; |
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return($self->{SCORE}); |
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} |
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=head2 get_start |
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Returns the start position of the feature. |
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=cut |
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sub get_start { |
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my $self = shift; |
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return($self->{START}); |
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} |
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=head2 get_end |
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Returns the end position of the feature. |
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=cut |
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sub get_end { |
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my $self = shift; |
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return($self->{END}); |
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} |
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162
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=head2 length |
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164
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Returns the length of the feature. |
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=cut |
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168
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sub length { |
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my $self = shift; |
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return($self->{END} - $self->{START} + 1); |
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} |
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=head2 get_seq |
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Returns the sequence of the feature. |
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=cut |
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sub get_seq { |
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my $self = shift; |
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return($self->{SEQ}); |
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} |
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184
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=head2 get_type |
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186
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Returns the type of the feature. (con, ssr, tfbs, rep, cpg, tss). |
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188
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con : evolutionary conserved non-coding region |
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ssr : simple sequence repeat |
190
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tfbs : transcription factor binding site |
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rep : repeat |
192
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cpg : cpg island |
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tss : transcription start site |
194
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195
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=cut |
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197
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sub get_type { |
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my $self = shift; |
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return($self->{TYPE}); |
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} |
201
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202
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=head2 get_motifid |
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204
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Returns the motif primary ID, if the feature type is "con". |
205
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206
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=cut |
207
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208
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sub get_motifid { |
209
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my $self = shift; |
210
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0
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return($self->{MOTIFID}); |
211
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} |
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213
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=head2 get_tfbsid |
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215
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Returns the tfbs primary ID, if the feature type is "tfbs". |
216
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217
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=cut |
218
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219
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sub get_tfbsid { |
220
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0
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1
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my $self = shift; |
221
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0
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return($self->{TFBSID}); |
222
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} |
223
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224
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=head2 get_cpgid |
225
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226
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Returns the cpg primary ID, if the feature type is "cpg". |
227
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228
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=cut |
229
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230
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sub get_cpgid { |
231
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0
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0
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1
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my $self = shift; |
232
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0
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return($self->{CPGID}); |
233
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} |
234
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235
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=head2 get_repeatid |
236
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237
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Returns the repeat primary ID, if the feature type is "rep". |
238
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239
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=cut |
240
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241
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sub get_repeatid { |
242
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0
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0
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1
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|
my $self = shift; |
243
|
0
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return($self->{REPEATID}); |
244
|
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} |
245
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246
|
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=head2 get_ssrid |
247
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248
|
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|
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|
Returns the ssr primary ID, if the feature type is "ssr". |
249
|
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250
|
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|
|
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|
=cut |
251
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252
|
|
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|
|
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sub get_ssrid { |
253
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
254
|
0
|
|
|
|
|
|
return($self->{SSRID}); |
255
|
|
|
|
|
|
|
} |
256
|
|
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|
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|
257
|
|
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|
|
|
|
=head2 get_tssid |
258
|
|
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|
259
|
|
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|
|
|
|
Returns the tss primary ID, if the feature type is "tss". |
260
|
|
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|
|
|
|
|
261
|
|
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|
|
|
|
=cut |
262
|
|
|
|
|
|
|
|
263
|
|
|
|
|
|
|
sub get_tssid { |
264
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
265
|
0
|
|
|
|
|
|
return($self->{TSSID}); |
266
|
|
|
|
|
|
|
} |
267
|
|
|
|
|
|
|
|
268
|
|
|
|
|
|
|
=head2 get_seqid |
269
|
|
|
|
|
|
|
|
270
|
|
|
|
|
|
|
Returns the primary ID of the sequence containing this feature. |
271
|
|
|
|
|
|
|
|
272
|
|
|
|
|
|
|
=cut |
273
|
|
|
|
|
|
|
|
274
|
|
|
|
|
|
|
sub get_seqid { |
275
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
276
|
0
|
|
|
|
|
|
return($self->{SEQ_ID}); |
277
|
|
|
|
|
|
|
} |
278
|
|
|
|
|
|
|
|
279
|
|
|
|
|
|
|
=head2 get_subsetid |
280
|
|
|
|
|
|
|
|
281
|
|
|
|
|
|
|
Returns the subset primary ID of the feature, if the feature type is "con". |
282
|
|
|
|
|
|
|
|
283
|
|
|
|
|
|
|
=cut |
284
|
|
|
|
|
|
|
|
285
|
|
|
|
|
|
|
sub get_subsetid { |
286
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
287
|
0
|
|
|
|
|
|
return($self->{SUBSET_ID}); |
288
|
|
|
|
|
|
|
} |
289
|
|
|
|
|
|
|
|
290
|
|
|
|
|
|
|
=head2 get_motif |
291
|
|
|
|
|
|
|
|
292
|
|
|
|
|
|
|
Returns the motif object associated with the feature. |
293
|
|
|
|
|
|
|
If the feature type is not "con" this value is NULL. |
294
|
|
|
|
|
|
|
|
295
|
|
|
|
|
|
|
=cut |
296
|
|
|
|
|
|
|
|
297
|
|
|
|
|
|
|
sub get_motif { |
298
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
299
|
0
|
|
|
|
|
|
return($self->{MOTIF}); |
300
|
|
|
|
|
|
|
} |
301
|
|
|
|
|
|
|
|
302
|
|
|
|
|
|
|
=head2 get_tss_type |
303
|
|
|
|
|
|
|
|
304
|
|
|
|
|
|
|
Returns the tss type, if the feature type is "tss". |
305
|
|
|
|
|
|
|
|
306
|
|
|
|
|
|
|
=cut |
307
|
|
|
|
|
|
|
|
308
|
|
|
|
|
|
|
sub get_tss_type { |
309
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
310
|
0
|
|
|
|
|
|
return($self->{T_TYPE}); |
311
|
|
|
|
|
|
|
} |
312
|
|
|
|
|
|
|
|
313
|
|
|
|
|
|
|
=head2 get_tss_id |
314
|
|
|
|
|
|
|
|
315
|
|
|
|
|
|
|
Returns the tss id, if the feature type is "tss". |
316
|
|
|
|
|
|
|
|
317
|
|
|
|
|
|
|
=cut |
318
|
|
|
|
|
|
|
|
319
|
|
|
|
|
|
|
sub get_tss_id { |
320
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
321
|
0
|
|
|
|
|
|
return($self->{T_ID}); |
322
|
|
|
|
|
|
|
} |
323
|
|
|
|
|
|
|
|
324
|
|
|
|
|
|
|
=head2 get_tss_desc |
325
|
|
|
|
|
|
|
|
326
|
|
|
|
|
|
|
Returns the description of the tss, if the feature type is "tss". |
327
|
|
|
|
|
|
|
|
328
|
|
|
|
|
|
|
=cut |
329
|
|
|
|
|
|
|
|
330
|
|
|
|
|
|
|
sub get_tss_desc { |
331
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
332
|
0
|
|
|
|
|
|
return($self->{T_DESC}); |
333
|
|
|
|
|
|
|
} |
334
|
|
|
|
|
|
|
|
335
|
|
|
|
|
|
|
=head2 get_tss_xref |
336
|
|
|
|
|
|
|
|
337
|
|
|
|
|
|
|
Returns the xref of the tss, if the feature type is "tss". |
338
|
|
|
|
|
|
|
|
339
|
|
|
|
|
|
|
=cut |
340
|
|
|
|
|
|
|
|
341
|
|
|
|
|
|
|
sub get_tss_xref { |
342
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
343
|
0
|
|
|
|
|
|
return($self->{T_XREF}); |
344
|
|
|
|
|
|
|
} |
345
|
|
|
|
|
|
|
|
346
|
|
|
|
|
|
|
1; |