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# BioPerl module for Bio::DB::EMBL |
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# Please direct questions and support issues to |
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# |
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# Cared for by Heikki Lehvaslaiho |
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# |
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# Copyright Jason Stajich |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::DB::EMBL - Database object interface for EMBL entry retrieval |
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=head1 SYNOPSIS |
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use Bio::DB::EMBL; |
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$embl = Bio::DB::EMBL->new(); |
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# remember that EMBL_ID does not equal GenBank_ID! |
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$seq = $embl->get_Seq_by_id('HSFOS'); # EMBL ID |
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print "cloneid is ", $seq->id, "\n"; |
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# or changeing to accession number and Fasta format ... |
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$embl->request_format('fasta'); |
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$seq = $embl->get_Seq_by_acc('J02231'); # EMBL ACC |
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print "cloneid is ", $seq->id, "\n"; |
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# especially when using versions, you better be prepared |
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# in not getting what what want |
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eval { |
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$seq = $embl->get_Seq_by_version('J02231.1'); # EMBL VERSION |
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}; |
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print "cloneid is ", $seq->id, "\n" unless $@; |
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# or ... best when downloading very large files, prevents |
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# keeping all of the file in memory |
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# also don't want features, just sequence so let's save bandwidth |
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# and request Fasta sequence |
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$embl = Bio::DB::EMBL->new(-retrievaltype => 'tempfile' , |
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-format => 'fasta'); |
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my $seqio = $embl->get_Stream_by_id(['AC013798', 'AC021953'] ); |
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while( my $clone = $seqio->next_seq ) { |
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print "cloneid is ", $clone->id, "\n"; |
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} |
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=head1 DESCRIPTION |
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Allows the dynamic retrieval of sequence objects L from the |
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EMBL database using the dbfetch script at EBI: |
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L. |
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In order to make changes transparent we have host type (currently only |
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ebi) and location (defaults to ebi) separated out. This allows later |
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additions of more servers in different geographical locations. |
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The functionality of this module is inherited from L |
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which implements L. |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to one |
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of the Bioperl mailing lists. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR - Heikki Lehvaslaiho |
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Email Heikki Lehvaslaiho Eheikki-at-bioperl-dot-orgE |
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=head1 APPENDIX |
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The rest of the documentation details each of the object |
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methods. Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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package Bio::DB::EMBL; |
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$Bio::DB::EMBL::VERSION = '1.7.3'; |
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use strict; |
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use vars qw($MODVERSION %HOSTS %FORMATMAP $DEFAULTFORMAT); |
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$MODVERSION = '0.2'; |
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use base qw(Bio::DB::DBFetch); |
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BEGIN { |
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# you can add your own here theoretically. |
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%HOSTS = ( |
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'dbfetch' => { |
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baseurl => 'http://%s/Tools/dbfetch/dbfetch?db=embl&style=raw', |
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hosts => { |
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'ebi' => 'www.ebi.ac.uk' |
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} |
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} |
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); |
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%FORMATMAP = ( 'embl' => 'embl', |
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'fasta' => 'fasta' |
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); |
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$DEFAULTFORMAT = 'embl'; |
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} |
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=head2 new |
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Title : new |
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Usage : $gb = Bio::DB::GenBank->new(@options) |
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Function: Creates a new genbank handle |
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Returns : New genbank handle |
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Args : -delay number of seconds to delay between fetches (3s) |
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NOTE: There are other options that are used internally. |
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=cut |
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sub new { |
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my ($class, @args ) = @_; |
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my $self = $class->SUPER::new(@args); |
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$self->{ '_hosts' } = {}; |
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$self->{ '_formatmap' } = {}; |
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$self->hosts(\%HOSTS); |
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$self->formatmap(\%FORMATMAP); |
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$self->{'_default_format'} = $DEFAULTFORMAT; |
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return $self; |
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} |
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1; |