| line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
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1
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package App::RL::Common; |
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2
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15
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15
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14614
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use strict; |
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15
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31
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15
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408
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3
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15
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15
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83
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use warnings; |
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15
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31
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15
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394
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4
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15
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15
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946
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use autodie; |
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15
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24833
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15
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82
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5
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6
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15
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15
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78714
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use 5.010001; |
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15
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104
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7
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8
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15
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15
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114
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use Carp qw(); |
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15
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32
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15
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271
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9
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15
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15
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8615
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use IO::Zlib; |
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15
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774017
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15
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96
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10
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15
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15
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807
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use List::Util qw(); |
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15
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34
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15
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245
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11
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15
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15
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9599
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use Path::Tiny qw(); |
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15
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119100
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15
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396
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12
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15
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15
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6528
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use Set::Scalar; |
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15
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123117
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15
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600
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13
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15
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15
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6016
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use Tie::IxHash; |
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15
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31781
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15
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397
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14
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15
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15
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5665
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use YAML::Syck qw(); |
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15
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21604
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15
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319
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15
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16
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15
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15
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6589
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use AlignDB::IntSpan; |
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15
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77227
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15
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16282
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17
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18
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# The only entrance for AlignDB::IntSpan or AlignDB::IntSpanXS |
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19
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#@returns AlignDB::IntSpan |
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20
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sub new_set { |
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21
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341
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341
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0
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1217
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return AlignDB::IntSpan->new; |
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22
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} |
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23
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24
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sub read_lines { |
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25
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13
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13
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0
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34
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my $fn = shift; |
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26
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27
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13
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37
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my @lines; |
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28
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29
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13
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50
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59
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if ( lc $fn eq "stdin" ) { |
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30
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0
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0
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while () { |
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31
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0
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0
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chomp; |
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32
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0
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0
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push @lines, $_; |
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33
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} |
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34
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} |
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35
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else { |
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36
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13
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78
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open my $fh, "<", $fn; |
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37
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13
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12816
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while (<$fh>) { |
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38
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140
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340
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chomp; |
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39
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140
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430
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push @lines, $_; |
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40
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} |
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41
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13
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64
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close $fh; |
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42
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} |
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43
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44
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13
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6441
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return @lines; |
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45
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} |
|
46
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47
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sub read_sizes { |
|
48
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9
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9
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0
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26
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my $fn = shift; |
|
49
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9
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27
|
my $remove_chr = shift; |
|
50
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51
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9
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44
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my @lines = read_lines($fn); |
|
52
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9
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53
|
my %length_of; |
|
53
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9
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31
|
for (@lines) { |
|
54
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122
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338
|
my ( $key, $value ) = split /\t/; |
|
55
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122
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50
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265
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$key =~ s/chr0?//i if $remove_chr; |
|
56
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122
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315
|
$length_of{$key} = $value; |
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57
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} |
|
58
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59
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9
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|
48
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return \%length_of; |
|
60
|
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} |
|
61
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62
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sub read_names { |
|
63
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1
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|
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1
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0
|
4
|
my $fn = shift; |
|
64
|
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65
|
1
|
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6
|
my @lines = read_lines($fn); |
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66
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67
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1
|
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|
9
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return \@lines; |
|
68
|
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} |
|
69
|
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70
|
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sub runlist2set { |
|
71
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57
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57
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0
|
5991
|
my $runlist_of = shift; |
|
72
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57
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128
|
my $remove_chr = shift; |
|
73
|
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74
|
57
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111
|
my $set_of = {}; |
|
75
|
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76
|
57
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133
|
for my $chr ( sort keys %{$runlist_of} ) { |
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57
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309
|
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77
|
225
|
|
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434
|
my $new_chr = $chr; |
|
78
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225
|
50
|
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|
605
|
$new_chr =~ s/chr0?//i if $remove_chr; |
|
79
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225
|
|
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|
|
611
|
my $set = new_set(); |
|
80
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225
|
|
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|
3073
|
$set->add( $runlist_of->{$chr} ); |
|
81
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225
|
|
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|
|
952296
|
$set_of->{$new_chr} = $set; |
|
82
|
|
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|
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} |
|
83
|
|
|
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84
|
57
|
|
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|
246
|
return $set_of; |
|
85
|
|
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|
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|
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} |
|
86
|
|
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|
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|
|
87
|
|
|
|
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|
|
sub decode_header { |
|
88
|
39
|
|
|
39
|
0
|
4473
|
my $header = shift; |
|
89
|
|
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|
|
|
|
90
|
39
|
|
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|
|
211
|
tie my %info, "Tie::IxHash"; |
|
91
|
|
|
|
|
|
|
|
|
92
|
|
|
|
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|
|
# S288.chrI(+):27070-29557|species=S288C |
|
93
|
39
|
|
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|
|
691
|
my $head_qr = qr{ |
|
94
|
|
|
|
|
|
|
(?:(?P[\w_]+)\.)? |
|
95
|
|
|
|
|
|
|
(?P[\w-]+) |
|
96
|
|
|
|
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|
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(?:\((?P.+)\))? |
|
97
|
|
|
|
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|
|
[\:] # spacer |
|
98
|
|
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|
|
|
|
(?P\d+) |
|
99
|
|
|
|
|
|
|
[\_\-]? # spacer |
|
100
|
|
|
|
|
|
|
(?P\d+)? |
|
101
|
|
|
|
|
|
|
}xi; |
|
102
|
|
|
|
|
|
|
|
|
103
|
39
|
|
|
|
|
346
|
$header =~ $head_qr; |
|
104
|
39
|
|
|
|
|
142
|
my $chr_name = $2; |
|
105
|
39
|
|
|
|
|
91
|
my $chr_start = $4; |
|
106
|
39
|
|
|
|
|
77
|
my $chr_end = $5; |
|
107
|
|
|
|
|
|
|
|
|
108
|
39
|
100
|
66
|
|
|
198
|
if ( defined $chr_name and defined $chr_start ) { |
|
109
|
36
|
100
|
|
|
|
96
|
if ( !defined $chr_end ) { |
|
110
|
1
|
|
|
|
|
3
|
$chr_end = $chr_start; |
|
111
|
|
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|
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|
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} |
|
112
|
|
|
|
|
|
|
%info = ( |
|
113
|
36
|
|
|
|
|
282
|
name => $1, |
|
114
|
|
|
|
|
|
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chr => $chr_name, |
|
115
|
|
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|
|
|
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strand => $3, |
|
116
|
|
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|
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|
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start => $chr_start, |
|
117
|
|
|
|
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|
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end => $chr_end, |
|
118
|
|
|
|
|
|
|
); |
|
119
|
36
|
100
|
|
|
|
2830
|
if ( defined $info{strand} ) { |
|
120
|
16
|
50
|
|
|
|
141
|
if ( $info{strand} eq '1' ) { |
|
|
|
50
|
|
|
|
|
|
|
121
|
0
|
|
|
|
|
0
|
$info{strand} = '+'; |
|
122
|
|
|
|
|
|
|
} |
|
123
|
|
|
|
|
|
|
elsif ( $info{strand} eq '-1' ) { |
|
124
|
0
|
|
|
|
|
0
|
$info{strand} = '-'; |
|
125
|
|
|
|
|
|
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} |
|
126
|
|
|
|
|
|
|
} |
|
127
|
|
|
|
|
|
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} |
|
128
|
|
|
|
|
|
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else { |
|
129
|
3
|
|
|
|
|
9
|
$header =~ /^(\S+)/; |
|
130
|
3
|
|
|
|
|
7
|
my $chr = $1; |
|
131
|
3
|
|
|
|
|
15
|
%info = ( |
|
132
|
|
|
|
|
|
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name => undef, |
|
133
|
|
|
|
|
|
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chr => $chr, |
|
134
|
|
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|
|
|
|
strand => undef, |
|
135
|
|
|
|
|
|
|
start => undef, |
|
136
|
|
|
|
|
|
|
end => undef, |
|
137
|
|
|
|
|
|
|
); |
|
138
|
|
|
|
|
|
|
} |
|
139
|
|
|
|
|
|
|
|
|
140
|
|
|
|
|
|
|
# additional keys |
|
141
|
39
|
100
|
|
|
|
633
|
if ( $header =~ /\|(.+)/ ) { |
|
142
|
1
|
|
|
|
|
6
|
my @parts = grep {defined} split /;/, $1; |
|
|
1
|
|
|
|
|
5
|
|
|
143
|
1
|
|
|
|
|
3
|
for my $part (@parts) { |
|
144
|
1
|
|
|
|
|
4
|
my ( $key, $value ) = split /=/, $part; |
|
145
|
1
|
50
|
33
|
|
|
12
|
if ( defined $key and defined $value ) { |
|
146
|
1
|
|
|
|
|
5
|
$info{$key} = $value; |
|
147
|
|
|
|
|
|
|
} |
|
148
|
|
|
|
|
|
|
} |
|
149
|
|
|
|
|
|
|
} |
|
150
|
|
|
|
|
|
|
|
|
151
|
39
|
|
|
|
|
162
|
return \%info; |
|
152
|
|
|
|
|
|
|
} |
|
153
|
|
|
|
|
|
|
|
|
154
|
|
|
|
|
|
|
sub info_is_valid { |
|
155
|
42
|
|
|
42
|
0
|
88
|
my $info = shift; |
|
156
|
|
|
|
|
|
|
|
|
157
|
42
|
50
|
|
|
|
138
|
if ( ref $info eq "HASH" ) { |
|
158
|
42
|
50
|
33
|
|
|
153
|
if ( exists $info->{chr} and exists $info->{start} ) { |
|
159
|
42
|
50
|
33
|
|
|
413
|
if ( defined $info->{chr} and defined $info->{start} ) { |
|
160
|
42
|
|
|
|
|
626
|
return 1; |
|
161
|
|
|
|
|
|
|
} |
|
162
|
|
|
|
|
|
|
} |
|
163
|
|
|
|
|
|
|
} |
|
164
|
|
|
|
|
|
|
|
|
165
|
0
|
|
|
|
|
0
|
return 0; |
|
166
|
|
|
|
|
|
|
} |
|
167
|
|
|
|
|
|
|
|
|
168
|
|
|
|
|
|
|
sub encode_header { |
|
169
|
19
|
|
|
19
|
0
|
31388
|
my $info = shift; |
|
170
|
19
|
|
|
|
|
48
|
my $only_essential = shift; |
|
171
|
|
|
|
|
|
|
|
|
172
|
19
|
|
|
|
|
33
|
my $header; |
|
173
|
19
|
100
|
|
|
|
113
|
if ( defined $info->{name} ) { |
|
|
|
50
|
|
|
|
|
|
|
174
|
7
|
50
|
|
|
|
46
|
if ( defined $info->{chr} ) { |
|
175
|
7
|
|
|
|
|
39
|
$header .= $info->{name}; |
|
176
|
7
|
|
|
|
|
38
|
$header .= "." . $info->{chr}; |
|
177
|
|
|
|
|
|
|
} |
|
178
|
|
|
|
|
|
|
else { |
|
179
|
0
|
|
|
|
|
0
|
$header .= $info->{name}; |
|
180
|
|
|
|
|
|
|
} |
|
181
|
|
|
|
|
|
|
} |
|
182
|
|
|
|
|
|
|
elsif ( defined $info->{chr} ) { |
|
183
|
12
|
|
|
|
|
152
|
$header .= $info->{chr}; |
|
184
|
|
|
|
|
|
|
} |
|
185
|
|
|
|
|
|
|
|
|
186
|
19
|
100
|
|
|
|
152
|
if ( defined $info->{strand} ) { |
|
187
|
10
|
|
|
|
|
61
|
$header .= "(" . $info->{strand} . ")"; |
|
188
|
|
|
|
|
|
|
} |
|
189
|
19
|
50
|
|
|
|
121
|
if ( defined $info->{start} ) { |
|
190
|
19
|
|
|
|
|
114
|
$header .= ":" . $info->{start}; |
|
191
|
19
|
100
|
|
|
|
108
|
if ( $info->{end} != $info->{start} ) { |
|
192
|
17
|
|
|
|
|
165
|
$header .= "-" . $info->{end}; |
|
193
|
|
|
|
|
|
|
} |
|
194
|
|
|
|
|
|
|
} |
|
195
|
|
|
|
|
|
|
|
|
196
|
|
|
|
|
|
|
# additional keys |
|
197
|
19
|
50
|
|
|
|
131
|
if ( !$only_essential ) { |
|
198
|
19
|
|
|
|
|
46
|
my %essential = map { $_ => 1 } qw{name chr strand start end seq full_seq}; |
|
|
133
|
|
|
|
|
291
|
|
|
199
|
19
|
|
|
|
|
44
|
my @parts; |
|
200
|
19
|
|
|
|
|
32
|
for my $key ( sort keys %{$info} ) { |
|
|
19
|
|
|
|
|
124
|
|
|
201
|
92
|
100
|
|
|
|
623
|
if ( !$essential{$key} ) { |
|
202
|
2
|
|
|
|
|
7
|
push @parts, $key . "=" . $info->{$key}; |
|
203
|
|
|
|
|
|
|
} |
|
204
|
|
|
|
|
|
|
} |
|
205
|
19
|
100
|
|
|
|
80
|
if (@parts) { |
|
206
|
2
|
|
|
|
|
5
|
my $additional = join ";", @parts; |
|
207
|
2
|
|
|
|
|
8
|
$header .= "|" . $additional; |
|
208
|
|
|
|
|
|
|
} |
|
209
|
|
|
|
|
|
|
} |
|
210
|
|
|
|
|
|
|
|
|
211
|
19
|
|
|
|
|
108
|
return $header; |
|
212
|
|
|
|
|
|
|
} |
|
213
|
|
|
|
|
|
|
|
|
214
|
|
|
|
|
|
|
1; |